Cancer Immunology, Immunotherapy

, Volume 48, Issue 1, pp 56–61 | Cite as

CD95 Ligand expression enhances growth of murine renal cell carcinoma in vivo

  • Hiroaki Nishimatsu
  • Takumi Takeuchi
  • Tetsuo Ueki
  • Takahiro Kajiwara
  • Nobuo Moriyama
  • Toshimitsu Ishida
  • Baoxing Li
  • Tadao Kakizoe
  • Tadaichi Kitamura
ORIGINAL ARTICLE

Abstract

The CD95/CD95 ligand (CD95L) system plays an important role in the induction of lymphoid apoptosis and has been implicated in the suppression of immune responses. In this system, two murine CD95L-transfected renca clones and a control renca clone transfected only with the vector were implanted into the subcapsule of the left kidney of Balb/c and Balb/c nude mice. Both CD95L-expressing and control renca clones formed macroscopic tumors in all of the Balb/c and Balb/c nude hosts 14 days after implantation. Growth of tumors of murine CD95L-transfected renca cells was significantly better than that of control renca cells in Balb/c mice, while the growth advantage of CD95L transfectants was not observed in Balb/c nude mice. Lymphocytes underwent apoptosis mainly in the periphery of the CD95L-expressing tumors but not in control tumors grown in Balb/c mice, while lymphocytes undergoing apoptosis were not observed in CD95L-expressing tumors or in control tumors grown in Balb/c nude mice. Neutrophilic recruitment was rarely observed in CD95L-expressing or control tumors. CD95L expressed on renca cells possibly suppressed immune responses against renca tumors by inducing apoptosis of the infiltrating lymphocytes. However, CD95L-expressing renca cells did not form tumors in the renal subcapsule of allogeneic C3H/HeJ mice.

Key words CD95L FasL Renca Apoptosis Tumor growth 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • Hiroaki Nishimatsu
    • 1
  • Takumi Takeuchi
    • 1
  • Tetsuo Ueki
    • 1
  • Takahiro Kajiwara
    • 1
  • Nobuo Moriyama
    • 1
  • Toshimitsu Ishida
    • 1
  • Baoxing Li
    • 1
  • Tadao Kakizoe
    • 2
  • Tadaichi Kitamura
    • 1
  1. 1.Department of Urology, University of Tokyo, Faculty of Medicine, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan Tel.: +81-3-5800-8662, Fax: +81-3-3622-7958JP
  2. 2.National Cancer Center, Tokyo

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