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Cancer Immunology, Immunotherapy

, Volume 47, Issue 5, pp 257–264 | Cite as

Adoptive-transfer therapy of tumors with the tumor-specific primary cytotoxic T cells induced in vitro with the B7.1-transduced MCA205 cell line

  • Sun Jung Kim
  • Michel Sadelain
  • Jeong-Soon Lee
  • Roh Hyun Seong
  • Yeon-Sook Yun
  • Young Ju Jang
  • Hee Young Chung
ORIGINAL ARTICLE

Abstract

We show that the tumor-specific primary cytotoxic T lymphocytes (CTL) induced in vitro with the MCA205 fibrosarcoma cells transduced with the B7.1 (CD80) gene are highly effective in adoptive-transfer therapy of the parental tumors. The MCA205 fibrosarcoma cell line was transduced with the retroviral vectors encoding the B7.1 gene and tested for their efficiency as stimulators in short-term (5 days) mixed lymphocyte/tumor cell cultures with highly purified syngenic, unprimed T cells as responders. The induction of the CTL required the presence of a low dose of interleukin-2 (25 U/ml). The injection of the CTL prevented colony formation by the intravenously injected tumor cells in a lung colonization assay in which the CTL were injected after inoculation of tumor cells. We also showed that the adoptive transfer of the same T cells was effective in delaying the growth of the subcutaneously injected tumor cells. These results imply that the short-term mixed lymphocyte/tumor cell culture with the tumor cells transduced with the gene for the B7.1 costimulatory molecule is potentially a good source of CTL for adoptive-transfer therapy of tumors.

Key words Primary cytotoxic T lymphocytes B7.1 Tumors Adoptive transfer Immunotherapy 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • Sun Jung Kim
    • 1
  • Michel Sadelain
    • 2
  • Jeong-Soon Lee
    • 1
  • Roh Hyun Seong
    • 3
  • Yeon-Sook Yun
    • 1
  • Young Ju Jang
    • 4
  • Hee Young Chung
    • 1
  1. 1.Laboratory of Tumor Immunology, Korea Cancer Center Hospital, Nowon-Gu, Gongneung 2 Dong, 215-4, Seoul, 139-706, Korea e-mail: imm@kcchsun.kcch.re.kr Tel.: +82-2-970-1308, Fax: +82-2-977-0381KR
  2. 2.Department of Human Genetics, Memorial Sloan-Kettering Cancer Center, New York, NY10021, USAUS
  3. 3.Laboratory of Molecular Immunology, Genetic Engineering Research Institute, Seoul National University, Seoul, 151-742, KoreaKR
  4. 4.Laboratory of Immunology, Medical Science Research Institute, Ajou University, Suwon, 442-749, KoreaKR

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