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Cancer Immunology, Immunotherapy

, Volume 47, Issue 1, pp 13–20 | Cite as

Induction of anti-idiotypic humoral and cellular immune responses by a murine monoclonal antibody recognizing the ovarian carcinoma antigen CA125 encapsulated in biodegradable microspheres

  • Jie Ma
  • John Samuel
  • Glen S. Kwon
  • Antoine A. Noujaim
  • Ragupathy Madiyalakan
ORIGINAL ARTICLE

Abstract

The use of biodegradable poly(dl-lactic-co-glycolic acid) microspheres as a cancer vaccine delivery system for induction of anti-idiotypic responses was investigated using a murine monoclonal antibody B43.13 that recognizes the human ovarian cancer antigen CA125. Immunization of mice with mAb B43.13 encapsulated in poly(dl-lactic-co-glycolic acid) microspheres resulted in enhanced humoral and cellular immune responses compared with mAb B43.13 alone or mAb B43.13 mixed with microspheres. The antibody responses could be further enhanced by the co-encapsulation of mAb B43.13 with monophosphoryl lipid A, a non-toxic adjuvant, in microspheres. Anti-idiotypic humoral responses were shown to result in Ab2 antibodies mimicking the nominal antigen CA125 and Ab3 antibodies recognizing CA125. Further, microsphere delivery of mAb B43.13 also resulted in induction of T cell responses involving T2 cells reactive with mAb B43.13 epitopes and T3 cells recognizing CA125. These results indicate that microsphere delivery of Ab1 can induce both humoral and cellular anti-idiotypic responses relevant to cancer antigens. This raises the possibility of the use of such formulations for anti-idiotypic induction immunotherapy for cancer.

Key words Anti-idiotypic antibody Poly(dl-lactic-co-glycolic acid) Microspheres Cancer vaccine 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Jie Ma
    • 1
  • John Samuel
    • 2
  • Glen S. Kwon
    • 2
  • Antoine A. Noujaim
    • 1
  • Ragupathy Madiyalakan
    • 1
  1. 1.Research and Development, AltaRex Corp., 300, 8625-112 Street, Edmonton, Alberta, T6G 2E1, CanadaCA
  2. 2.Faculty of Pharmacy and Pharmaceutical Sciences, 3118 Dentistry Pharmacy Building, University of Alberta, Edmonton, Alberta, T6G 2N8, Canada e-mail: jsamuel@pharmacy.ualberta.ca Tel.: +1-403-492-7469, Fax: +1-403-492-1217CA

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