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Cancer Immunology, Immunotherapy

, Volume 43, Issue 2, pp 116–123 | Cite as

Immune response against medullary thyroid carcinoma (MTC) induced by parental and/or interleukin-2-secreting MTC cells in a rat model of human familial medullary thyroid carcinoma

  • S. Lausson
  • B. Fournes
  • C. Borrel
  • G. Milhaud
  • F. Treilhou-Lahille
ORIGINAL ARTICLE

Abstract

 The existence of inherited aggressive forms of medullary thyroid carcinoma (MTC), and their resistance to all classical therapies, make it a prime candidate for adoptive immunotherapy. As a prelude to a vaccine for the protection of family members at risk of developing the disease, we investigated the immunological antitumour response provoked by the 6/23 rMTC cell line, compared to that of the same cells engineered to secrete interleukin-2 (rMTC-IL2), in an animal model of familial human MTC, the inbred strain of Wag/Rij rats. The rMTC cells developed a tumour that invaded the whole neck 15 days after orthotopic injection (into the thyroid), while the rMTC-IL2 cells were progressively rejected. Co-injection of rMTC-IL2 with the parental cells induced the rejection of the rMTC transplants. When injected, both tumoral cell types showed a similar positive immunoreaction with anti-MHC class I (major histocompatibility complex class I) antibodies. They both recruited natural killer cells and eosinophils at the site of injection. In addition, CD8+ T lymphocytes infiltrated the rMTC-IL2 cells, and eosinophil recruitment was amplified. Neutrophils, macrophages and CD4+ T lymphocytes were scarce. Our results suggest that the CD8+ T lymphocytes are implicated in the antitumour reaction elicited by the Il-2-transfected cells. As these effectors are known to induce a specific immunological response, including memory, such a protocol should be tested as a vaccine on the young population genetically at risk of developing a MTC.

Key words Medullary thyroid carcinoma Rat Immunotherapy Interleukin-2 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • S. Lausson
    • 1
  • B. Fournes
    • 1
  • C. Borrel
    • 1
  • G. Milhaud
    • 2
  • F. Treilhou-Lahille
    • 1
  1. 1.Laboratoire d’Endocrinologie Cellulaire et Evolution, U. R. A. 1116 CNRS, Bât. 440, Université Paris-Sud, Centre d’Orsay, 91405 Orsay Cédex, FranceFR
  2. 2.Service de Biophysique, Hôpital Saint Antoine, 75012 Paris, FranceFR

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