Conventional CARs versus modular CARs
The clinical application of immune effector cells genetically modified to express chimeric antigen receptors (CARs) has shown impressive results including complete remissions of certain malignant hematological diseases. However, their application can also cause severe side effects such as cytokine release syndrome (CRS) or tumor lysis syndrome (TLS). One limitation of currently applied CAR T cells is their lack of regulation. Especially, an emergency shutdown of CAR T cells in case of life-threatening side effects is missing. Moreover, targeting of tumor-associated antigens (TAAs) that are not only expressed on tumor cells but also on vital tissues requires the possibility of a switch allowing to repeatedly turn the activity of CAR T cells on and off. Here we summarize the development of a modular CAR variant termed universal CAR (UniCAR) system that promises to overcome these limitations of conventional CARs.
KeywordsImmunotherapy Chimeric antigen receptor T cells UniCAR BiTE TIMO XIV
American Society of Hematology
B-cell acute lymphoblastic leukemia
Bispecific T-cell engager
Chimeric antigen receptor(s)
Cluster of differentiation
Cytokine release syndrome
Diffuse large B-cell lymphoma
Epidermal growth factor receptor
U. S. Food and Drug Administration
Cell surface-associated mucin 1
Oklahoma Medical Research Foundation
Prostate stem cell antigen
Prostate-specific membrane antigen
Single-chain fragment variable
Tumor lysis syndrome
Universal, modular BiTE
Although their clinical efficacy is impressive, current CAR T cells are far from being a safe drug. After adoptive transfer, CAR T cells are confronted to numerous tumor cells expressing their target. As a consequence, the CAR T cells will be activated, proliferate and produce tremendous amounts of proinflammatory cytokines which can lead to serious CRS. In addition, the rapid disintegration of a huge amount of tumor cells can cause TLS. Furthermore, during anti-CD19 CAR T-cell treatments, deadly edema occurred in the central nervous system. In case the respective tumor cell target is not only overexpressed on tumor tissues but is also accessible on the surface of healthy tissues, CAR T cells will attack these healthy tissues as well which can cause life-threatening on-target/off-tumor effects [e.g., 18]. On-target/off-tumor effects can even occur during an anti-CD19 CAR T-cell therapy as CD19 is also expressed on the surface of healthy B cells. Fortunately, the loss of B cells is not life-threatening as the lack of antibodies (Abs) can be substituted. However, such a rescue is more or less unique for the target CD19 and not possible in case tumor-associated antigens (TAAs) are targeted that are accessible on the surface of vital tissues. To increase the safety of CAR T cells, a series of strategies has already been described including, e.g., the use of suicide genes, the introduction of an apoptosis switch with the CRISPR/Cas9 system, the application of inhibitory CARs, or the targeting of co-expressed surface antigens, e.g., a truncated version of epidermal growth factor receptor (EGFR) with Cetuximab [19, 20, 21, 22, 23]. The obvious task of all of these safeguards is the elimination of the CAR T cells. However, can such strategies work fast enough for the treatment of acute CRS or TLS or central nervous system problems? Most probably not; as recently summarized, full-size Abs or related recombinant derivatives require up to 48 h to be enriched in a solid tumor as is known from positron-emission tomography (PET) imaging [16, 17]. Even 2 h after application, the majority of mAb is still in the peripheral blood and only little is found in the tumor. Thus, a rapid steering with full-size mAbs appears quite unlikely. Similar time problems may occur when a genetic approach should be applied to destroy CAR T cells.
Nonetheless, all these approaches may be useful to protect healthy tissues that express cross-reactive epitopes against the attack of CAR T cells; if applied once, all tumor cells are destroyed. Indeed, anti-CD19 CAR T cells could be eliminated experimentally in mice , although it still might remain challenging to find the right time point for patients; obviously, if applied too late, on-target/off-tumor effects will occur. If eliminated too early, remaining tumor cells may lead to recurrent disease. Unfortunately, the sensitivity of currently available imaging technologies is not sufficient to detect few remaining tumor cells. Even in case improved imaging tools may become available, one still would not know about the sensitivity of the detectable tumor cells against the CAR T cells. It could easily be that the remaining tumor cells have downregulated the target recognized by the CAR T cells. For all these reasons, it would be much better not to eliminate the CAR T cells but to regulate their function, e.g., to equip CAR T cells with a rapid and reliable switch to adapt their activity and specificity in case tumor escape variants occur, and to target more than one TAA to reduce the risk for escape variants.
Modular CARs as solution
In 2012, Urbanska et al. described a modular artificial receptor approach. Instead of an anti-TAA Ab domain, the authors used chicken avidin as the extracellular receptor domain of the artificial receptor . T cells modified with such an artificial avidin receptor were able to target tumor cells via biotinylated adaptor molecules, e.g., biotinylated Abs. However, chicken avidin (or bacterial streptavidin) might be highly immunogenic in humans. Furthermore, the presence of natural anti-biotin Abs in sera of healthy individuals might limit the use of avidin-based receptors in humans . Bearing in mind that full-size Abs have a half-life of several weeks and require at least 24–48 h to enrich at the tumor site, one can expect that adaptor CARs armed with Abs behave more or less like conventional CARs with little chance of a rapid regulation. For these reasons, a rapid switch off, e.g., for the treatment of CRS might not work at least not with adaptor molecules based on full-size Abs. Moreover, if the target is also expressed on the cell surface of blood or endothelial cells, intravenously applied adaptor/CAR T-cell complexes might first attack these healthy cells rather than to leave the bloodstream to find the tumor cells.
So how to solve these problems? While we tried to establish novel bispecific Abs (bsAbs) [e.g., 26, 27, 28, 29, 30, 31], we learned that even minor changes in one of the two Ab domains did not only affect the altered Ab domain but also the non-modified one. To easily compare different anti-CD3 and anti-TAA domains, we created a modular bsAb format. For this purpose, we split the bsAb into two components: (1) an effector molecule (EM) and (2) a target molecule (TM). The original idea was to create a bsAb that can be used as a universal EM: for that purpose, on the one hand, the EM is directed to CD3 and, on the other hand, to a suitable peptide epitope. TMs were designed to consist of an anti-TAA scFv fused to the peptide epitope [e.g., 32, 33, 34]. Thus, EM and TM can form an immune complex. For proof of concept, we tested a series of peptide epitope tags including, e.g., the oligo(Histidine)-tag or the Myc-tag which were not functional. For several reasons including a low risk of immunogenicity we finally selected two peptide sequences termed E5B9 and E7B6. Both epitope sequences derive from the human nuclear autoantigen La also known as Sjögren’s syndrome-associated antigen B (SS-B) (for more details, see below and also ). Interestingly, we found that the respective EM/TM complexes can functionally replace conventional bsAbs [32, 33, 34]. This modular bispecific T-cell engager (BiTE) format was termed universal, modular BiTE system (UniMAB).
Experimental data for an anti-E5B9-based UniCAR system are presented in Fig. 3b–d. As shown, UniCAR T cells are able to specifically lyse target cells both in vitro (Fig. 3b, c) and in experimental mice (Fig. 3d). Tumor cell killing depends on functional UniCARs and on the presence of a TM: T cells transduced with UniCARs lacking a signaling domain are not able to kill tumor cells (Fig. 3,b), neither in the absence (Fig. 3b, gray bar) nor presence of a TM (Fig. 3b, black bar). Similarly, UniCAR T cells containing a signaling domain cannot kill tumor cells in the absence of a TM (Fig. 3c, gray bar). However, in the presence of a TM, UniCAR T cells containing the signaling domain are able to kill tumor cells (Fig. 3c, black bar).
For first clinical phase 1 trials, we have selected the anti-E5B9 UniCAR version. Between anti-E5B9 scFv and TMD, the extracellular domain of the anti-E5B9 CAR contains the E7B6 epitope sequence as spacer. This E7B6 tag can be used for detection and elimination of the anti-E5B9 UniCARs via anti-E7B6-directed UniCARs if it becomes necessary . In contrast to all other current approaches to destroy unwanted CAR T cells such a procedure would not just eliminate but in parallel replace the anti-E5B9 UniCAR system with the alternative anti-E7B6 UniCAR system.
The E5B9 UniCAR system was first presented at the American Society of Hematology (ASH) meeting in 2014  and already summarized in detail . Since then, other related switchable CAR strategies (e.g., sCARs) were published [44, 45, 46].
An obvious problem of such a treatment strategy is: to achieve a sufficiently high concentration of the TM in the patient, it must be applied by continuous infusion. As the treatment may take weeks such a regimen appears inconvenient for patients. However, one could apply a TM with a short half-life at the beginning of a UniCAR therapy, when the tumor burden is high and thus the risk of CRS and TLS might also be high. Once the majority of the tumor is destroyed and these risks are low, one could switch to the application of a TM with a longer half-life. As estimated by PET imaging, immunoglobulin G4 (IgG4)-based TMs have a kinetic comparable to full-size Abs, consequently, TMs based on an IgG backbone are promising candidates for such an application . Until now, we have developed a series of TMs both with short and extended half-lives including, e.g., against CD19, CD123, CD33, prostate stem cell antigen (PSCA), prostate-specific membrane antigen (PSMA), disialoganglioside 2 (GD2), EGFR, cell surface-associated mucin 1 (MUC1), sialyl-Tn (STn), and others [36-43, Bachmann, unpublished]. From these studies we know that TMs can be constructed in a variety of formats. They can be cloned as scFvs from the variable heavy- and light-chain domains of murine or humanized mAbs but also from camelid Abs, so-called nanobodies. Interestingly, TMs can also be prepared from affibodies and soluble T-cell receptors. Even small molecules can be converted into TMs . For example, we successfully created a TM against PSMA by fusion of a UniCAR epitope to a commonly used PSMA PET tracer. The resulting theranostic TM can be used for both retargeting of UniCAR T cells and PET imaging to follow the CAR T-cell therapy in an individualized manner including in humans . In addition to monovalent TMs, we also created bivalent or bispecific TMs. To reduce the risk of tumor escape variants different monospecific TMs or bispecific TMs can be applied either simultaneously or subsequently for (OR) gated targeting .
As mentioned above, both UniCAR epitopes were taken from the nuclear autoantigen La/SS-B [48, 49, 50, 51]. Both epitopes are cryptic in native La protein as the respective mAb does not coprecipitate native La protein, while synthetic peptides or epitope fusion proteins or denatured La protein reacts with the respective anti-La mAb. The E5B9 epitope consists of the amino acid (aa) sequence KPLPEVTDEY which represents the aa95–104 of the La protein. In the La protein, this peptide sequence is located between the N-terminal La motif and the first ribonucleoprotein consensus sequence. The E7B6 epitope consists of the aa EKEALKKIIEDQQESLNK which represents the aa311–328 of La protein. In native La protein, it forms a helical structure which is located in the C-terminal domain of La protein.
The major reason why we selected UniCARs based on the E5B9 epitope for first phase 1 clinical studies is: over the past decades, we and many other groups have analyzed the structure, function, expression, and also the immune response against the La antigen including in experimental mice and autoimmune patients [e.g., 48, 49, 50, 51, 52, 53]. In none of these studies, we saw an immune response against the selected La epitopes in anti-La-positive patients neither at the B nor T cell level. Consequently, even autoimmune patients being able to break tolerance against the La antigen do not develop anti-La Abs against the UniCAR epitope(s). Therefore, it appears rather unlikely that people being tolerant to the nuclear La protein will develop an immune response against the selected La epitopes. But even in the worst case that we would induce an autoimmune response against the UniCAR epitope, anti-La Abs have been reported to be protective against anti-DNA Abs in lupus patients . Another advantage of the selected UniCAR epitopes is that the primary sequence of La protein is conserved during evolution including in rodents , so the toxicology of UniCAR T cells can easily be studied in mice.
In summary, the UniCAR system may help to overcome safety issues of the current CAR technology especially in solid tumors.
We would like to thank all former and current Bachelor, Master, Diploma, PhD and MD students, Postdocs, and technicians for their enthusiastic support. Especially, we would like to acknowledge Prof. Dr. M. Schmitz, Dr. H. Bartsch, Prof. Dr. H. Abken, Prof. Dr. A. Temme, and Dr. M. Cartellieri, who helped us to establish the conventional CAR T-cell technology. We thank all our coworkers of the spin-out companies GEMoaB and Cellex PT for the development of clinical-grade GMP products based on our BiTE and the UniCAR system. Thanks also to all former colleagues at the Oklahoma Medical Research Foundation (OMRF), Drs. B. Kurien, K. Kaufman, Profs. Dr. H. Scofield, A. D. Farris, J. A. James, J. B. Harley, and especially T. and J. A. Gross who helped to characterize the anti-La mAbs. In addition, we would like to thank Prof. Dr. G. Ehninger for his friendship, enthusiastic effort and support to drive our BiTE and the UniCAR system forward into the clinic.
AF, CA, SK, NB, RB, and MB conceptualized this review. AF, CA and MB prepared the figures and wrote the manuscript. All authors discussed the content and approved the final version of the manuscript.
There is no funding directly related to this review article.
Compliance with ethical standards
Conflict of interest
M Bachmann had the idea and supervised the development of the modular BiTE and the UniCAR system and filed patents related to the anti-La mAbs, BiTEs and the UniCAR system. In addition, he is the founder and shareholder of the company GEMoaB which owns these patents. All the authors declare that there are no other conflicts of interest.
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