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Cancer Immunology, Immunotherapy

, Volume 64, Issue 4, pp 419–427 | Cite as

Increased proportion of FoxP3+ regulatory T cells in tumor infiltrating lymphocytes is associated with tumor recurrence and reduced survival in patients with glioblastoma

  • Elias J. Sayour
  • Pat McLendon
  • Roger McLendon
  • Gabriel De Leon
  • Renee Reynolds
  • Jesse Kresak
  • John H. Sampson
  • Duane A. MitchellEmail author
Original Article

Abstract

Glioblastoma multiforme (GBM) is an aggressive malignancy associated with profound host immunosuppression mediated in part by FoxP3 expressing regulatory CD4+ T lymphocytes (Tregs) that down-regulate anti-tumor immunity. In order to assess whether FoxP3 was an independent driver differentially expressed in primary versus recurrent GBMs, we stained resected primary and recurrent GBM tumors for CD3, CD4, CD8 and FoxP3 expression using standard immunohistochemistry. Slides were scanned with a high-resolution scanner (ScanScope CS; Aperio), and image analysis software (Aperio ScanScope) was used to enumerate lymphocyte subpopulations allowing for high-throughput analysis and bypassing manual selection bias. As shown in previous studies, enumeration of individual lymphocyte populations did not correlate with clinical outcomes in patients with GBM. However, the CD4+ to regulatory FoxP3+ T cell ratio was diminished in recurrent disease, and increased CD3 and CD8+ to regulatory T cell ratios showed a positive correlation with survival outcomes in primary GBM. These results suggest that while absolute numbers of tumor infiltrating lymphocytes may not be informative for predicting clinical outcomes in patients with GBM, the effective balance of CD3, CD4 and CD8+ T cells to immunosuppressive FoxP3+ regulatory cells may influence clinical outcomes in this patient population.

Keywords

Tumor infiltrating lymphocytes Regulatory T cells (Tregs) Primary and recurrent GBM Immunotherapy 

Abbreviations

GBM

Glioblastoma multiforme

H&E

Hematoxylin and eosin

IHC

Immunohistochemistry

PBMCs

Peripheral blood mononuclear cells

PPCv9

Positive pixel count v9

Tregs

Regulatory T lymphocytes

TILs

Tumor infiltrating lymphocytes

Notes

Acknowledgments

This work was supported by the National Institutes of Health (NIH)/National Cancer Institute/National Institute of Neurological Disorders and Stroke Grants [5R01-NS067037 (to D.A. Mitchell), 5R01-CA134844 (to D.A. Mitchell), 5P50-CA108786 (to J.H. Sampson/Bigner)] and a Clinical and Translational Science Awards Grant UL1RR024128 from the National Center for Research Resources, a component of the NIH and NIH Roadmap for Medical Research. Additional support was provided by Accelerate Brain Cancer Cure (ABC2), National Brain Tumor Society, the American Brain Tumor Association, and the Pediatric Brain Tumor Foundation of the United States.

Conflict of interest

The authors declare that they have no conflict of interest with the research presented in this paper.

Supplementary material

262_2014_1651_MOESM1_ESM.pdf (186 kb)
Supplementary material 1 (PDF 185 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Elias J. Sayour
    • 1
    • 2
  • Pat McLendon
    • 3
  • Roger McLendon
    • 2
  • Gabriel De Leon
    • 1
    • 4
  • Renee Reynolds
    • 3
  • Jesse Kresak
    • 5
  • John H. Sampson
    • 2
    • 3
  • Duane A. Mitchell
    • 1
    Email author
  1. 1.University of Florida Brain Tumor Immunotherapy Program, Preston A. Wells, Jr. Center for Brain Tumor Therapy, McKnight Brain Institute, Department of NeurosurgeryUniversity of FloridaGainesvilleUSA
  2. 2.Department of PathologyDuke University Medical CenterDurhamUSA
  3. 3.Duke Brain Tumor Immunotherapy Program, Division of Neurosurgery, Department of SurgeryDuke University Medical CenterDurhamUSA
  4. 4.Department of Molecular Cancer BiologyDuke University Medical CenterDurhamUSA
  5. 5.Department of PathologyUniversity of FloridaGainesvilleUSA

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