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Cancer Immunology, Immunotherapy

, Volume 63, Issue 6, pp 587–599 | Cite as

Salmonella-mediated tumor regression involves targeting of tumor myeloid suppressor cells causing a shift to M1-like phenotype and reduction in suppressive capacity

  • Suneesh Kaimala
  • Yassir A. Mohamed
  • Nancy Nader
  • Jincy Issac
  • Eyad Elkord
  • Salem Chouaib
  • Maria J. Fernandez-Cabezudo
  • Basel K. al-Ramadi
Original Article

Abstract

The effectiveness of attenuated Salmonella in inhibiting tumor growth has been demonstrated in many therapeutic models, but the precise mechanisms remain incompletely understood. In this study, we show that the anti-tumor capacity of Salmonella depends on a functional MyD88-TLR pathway and is independent of adaptive immune responses. Since myeloid suppressor cells play a critical role in tumor growth, we investigated the consequences of Salmonella treatment on myeloid cell recruitment, phenotypic characteristics, and functional activation in spleen and tumor tissue of B16.F1 melanoma-bearing mice. Salmonella treatment led to increased accumulation of splenic and intratumoral CD11b+Gr-1+ myeloid cells, exhibiting significantly increased expression of various activation markers such as MHC class II, costimulatory molecules, and Sca-1/Ly6A proteins. Gene expression analysis showed that Salmonella treatment induced expression of iNOS, arginase-1 (ARG1), and IFN-γ in the spleen, but down-regulated IL-4 and TGF-β. Within the tumor, expression of iNOS, IFN-γ, and S100A9 was markedly increased, but ARG1, IL-4, TGF-β, and VEGF were inhibited. Functionally, splenic CD11b+ cells maintained their suppressive capacity following Salmonella treatment, but intratumoral myeloid cells had significantly reduced suppressive capacity. Our findings demonstrate that administration of attenuated Salmonella leads to phenotypic and functional maturation of intratumoral myeloid cells making them less suppressive and hence enhancing the host’s anti-tumor immune response. Modalities that inhibit myeloid suppressor cells may be useful adjuncts in cancer immunotherapy.

Keywords

Myeloid suppressor cells Salmonella Tumor immunity Macrophages 

Notes

Acknowledgments

We thank Dr. Toby Eisenstein (Temple University School of Medicine, Philadelphia, USA) for critical review of the manuscript. We are grateful to Dr. Samir Attoub (Department of Pharmacology, College of Medicine & Health Sciences, United Arab Emirates University) for providing us with the NMRI/nude mice. We also thank Arshad Khan for animal care and husbandry. This work was funded by grants from the Terry Fox Fund for Cancer Research and the UAE University-NRF (to B. K. al-Ramadi).

Conflict of interest

The authors declare no competing interests.

Supplementary material

262_2014_1543_MOESM1_ESM.pdf (648 kb)
Supplementary material 1 (PDF 648 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Suneesh Kaimala
    • 1
  • Yassir A. Mohamed
    • 1
  • Nancy Nader
    • 1
  • Jincy Issac
    • 1
  • Eyad Elkord
    • 1
  • Salem Chouaib
    • 3
  • Maria J. Fernandez-Cabezudo
    • 2
  • Basel K. al-Ramadi
    • 1
  1. 1.Department of Medical Microbiology and Immunology, College of Medicine and Health SciencesUnited Arab Emirates UniversityAl AinUnited Arab Emirates
  2. 2.Department of Biochemistry, College of Medicine and Health SciencesUnited Arab Emirates UniversityAl AinUnited Arab Emirates
  3. 3.Institut National de la Sante et de la Recherche Medicale, Unite 753Institut Gustave RoussyVillejuifFrance

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