Cancer Immunology, Immunotherapy

, Volume 62, Issue 10, pp 1609–1618

Reduced expression of NGEP is associated with high-grade prostate cancers: a tissue microarray analysis

  • Monireh Mohsenzadegan
  • Zahra Madjd
  • Mojgan Asgari
  • Maryam Abolhasani
  • Mehdi Shekarabi
  • Jaleh Taeb
  • Ahmad Shariftabrizi
Original Article


New gene expressed in prostate (NGEP) is a newly diagnosed prostate-specific gene that is expressed only in normal prostate and prostate cancer cells. Discovery of tissue-specific markers may promote the development of novel targets for immunotherapy of prostate cancer. In the present study, the staining pattern and clinical significance of NGEP were evaluated in a series of prostate tissues composed of 123 prostate cancer, 19 high-grade prostatic intraepithelial neoplasia and 44 samples of benign prostate tissue included in tissue microarrays using immunohistochemistry. Our study demonstrated that NGEP localized mainly in the apical and lateral membranes and was also partially distributed in the cytoplasm of epithelial cells of normal prostate tissue. All of the examined prostate tissues expressed NGEP with a variety of intensities; the level of expression was significantly more in the benign prostate tissues compared to malignant prostate samples (P value <0.001). Among prostate adenocarcinoma samples, a significant and inverse correlation was observed between the intensity of NGEP expression and increased Gleason score (P = 0.007). Taken together, we found that NGEP protein is widely expressed in low-grade to high-grade prostate adenocarcinomas as well as benign prostate tissues, and the intensity of expression is inversely proportional to the level of malignancy. NGEP could be an attractive target for immune-based therapy of prostate cancer patients as an alternative to the conventional therapies particularly in indolent patients.


NGEP Prostate cancer Immunotherapy Immunohistochemistry 


Ano 7

Anoctamin 7


Benign prostatic hyperplasia


Extraprostatic extension


Expressed sequence tags


Food and drug administration


High-grade prostatic intraepithelial neoplasia




In situ hybridization


Monoclonal antibody


New gene expressed in prostate


Peripheral blood mononuclear cell


Prostate cancer


Prostate-specific antigen


Prostate-specific membrane antigen


Prostate stem cell antigen


Tissue macroarray


Triad of costimulatory molecules (B7.1, ICAM-1, LFA-3)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Monireh Mohsenzadegan
    • 1
    • 2
  • Zahra Madjd
    • 3
    • 5
  • Mojgan Asgari
    • 3
    • 4
  • Maryam Abolhasani
    • 3
    • 4
  • Mehdi Shekarabi
    • 2
  • Jaleh Taeb
    • 5
  • Ahmad Shariftabrizi
    • 3
    • 6
  1. 1.Immunology Research CentreIran University of Medical SciencesTehranIran
  2. 2.Department of ImmunologyIran University of Medical SciencesTehranIran
  3. 3.Department of Pathology, Oncopathology Research CentreIran University of Medical SciencesTehranIran
  4. 4.Department of Pathology, Hasheminejad Kidney CenterIran University of Medical SciencesTehranIran
  5. 5.Department of Molecular MedicineIran University of Medical SciencesTehranIran
  6. 6.Department of Pathology, Danbury HospitalYale UniversityDanburyUSA

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