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Cancer Immunology, Immunotherapy

, Volume 62, Issue 2, pp 299–307 | Cite as

Comparative analysis of monocytic and granulocytic myeloid-derived suppressor cell subsets in patients with gastrointestinal malignancies

  • Austin Duffy
  • Fei Zhao
  • Lydia Haile
  • Jaba Gamrekelashvili
  • Suzanne Fioravanti
  • Chi Ma
  • Tamar Kapanadze
  • Kathryn Compton
  • William D. Figg
  • Tim F. GretenEmail author
Original Article

Abstract

Myeloid-derived suppressor cells (MDSC) are a heterogenous population of cells comprising myeloid progenitor cells and immature myeloid cells, which have the ability to suppress the effector immune response. In humans, MDSC have not been well characterized owing to the lack of specific markers, although it is possible to broadly classify the MDSC phenotypes described in the literature as being predominantly granulocytic (expressing markers such as CD15, CD66, CD33) or monocytic (expressing CD14). In this study, we set out to perform a direct comparative analysis across both granulocytic and monocytic MDSC subsets in terms of their frequency, absolute number, and function in the peripheral blood of patients with advanced GI cancer. We also set out to determine the optimal method of sample processing given that this is an additional source of heterogeneity. Our findings demonstrate consistent changes across sample processing methods for monocytic MDSC, suggesting that reliance upon cryopreserved PBMC is acceptable. Although we did not see an increase in the population of granulocytic MDSC, these cells were found to be more suppressive than their monocytic counterparts.

Keywords

Myeloid-derived suppressor cells Immune Suppressor Cancer 

Notes

Acknowledgments

This research was supported by the Intramural Research Program of the NIH, National Cancer Institute, Center for Cancer Research.

Conflict of interest

The authors declare no conflict of interest.

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Copyright information

© Springer-Verlag (outside the USA) 2012

Authors and Affiliations

  • Austin Duffy
    • 1
  • Fei Zhao
    • 1
  • Lydia Haile
    • 1
  • Jaba Gamrekelashvili
    • 1
  • Suzanne Fioravanti
    • 1
  • Chi Ma
    • 1
  • Tamar Kapanadze
    • 1
  • Kathryn Compton
    • 2
  • William D. Figg
    • 1
  • Tim F. Greten
    • 1
    Email author
  1. 1.GI Malignancy Section, Medical Oncology Branch, Center for Cancer ResearchNational Cancer InstituteBethesdaUSA
  2. 2.SAIC-Frederick, Inc.National Cancer InstituteBethesdaUSA

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