Cancer Immunology, Immunotherapy

, Volume 61, Issue 4, pp 581–585 | Cite as

The entirely carbohydrate immunogen Tn-PS A1 induces a cancer cell selective immune response and cytokine IL-17

  • Ravindra A. De Silva
  • Dananjaya K. Appulage
  • Halina Pietraszkiewicz
  • Kevin R. Bobbitt
  • Joe Media
  • JiaJiu Shaw
  • Fred A. Valeriote
  • Peter R. Andreana
Short Communication

Abstract

The tumor-associated carbohydrate antigen/hapten Thomsen-nouveau (Tn; α-D-GalpNAc-ONH2) was conjugated to a zwitterionic capsular polysaccharide, PS A1, from commensal anaerobe Bacteroides fragilis ATCC 25285/NCTC 9343 for the development of an entirely carbohydrate cancer vaccine construct and probed for immunogenicity. This communication discloses that murine anti-Tn IgG3 antibodies both bind to and recognize human tumor cells that display the Tn hapten. Furthermore, the sera from immunization of mice with Tn-PS A1 contain cytokine interleukin 17 (IL-17A), which is known to possess anti-tumor function and represents a striking difference to an IL-2, and IL-6 profile obtained with anti-PS A1 sera.

Keywords

Carbohydrates Antigen Tumor Vaccine Interleukin 17 Flow cytometry 

Notes

Acknowledgments

Authors thank Dr. Mary Kay Pflum for Jurkat and JurkatTAg cell lines. P.R.A acknowledges Wayne State University for financial support and the National Institutes of Health/National Cancer Institute for an R01 CA 156661 award.

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Ravindra A. De Silva
    • 1
  • Dananjaya K. Appulage
    • 1
  • Halina Pietraszkiewicz
    • 2
    • 3
  • Kevin R. Bobbitt
    • 4
  • Joe Media
    • 2
    • 3
  • JiaJiu Shaw
    • 5
  • Fred A. Valeriote
    • 2
    • 3
  • Peter R. Andreana
    • 1
  1. 1.Department of ChemistryWayne State UniversityDetroitUSA
  2. 2.Division of Hematology and Oncology, Department of Internal MedicineHenry Ford Health SystemDetroitUSA
  3. 3.The Josephine Ford Cancer CenterDetroitUSA
  4. 4.Department of Public Health Sciences Henry Ford HospitalDetroitUSA
  5. 5.21st Century Therapeutics, Inc.DetroitUSA

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