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Cancer Immunology, Immunotherapy

, Volume 60, Issue 4, pp 479–485 | Cite as

Gc protein-derived macrophage-activating factor (GcMAF) stimulates cAMP formation in human mononuclear cells and inhibits angiogenesis in chick embryo chorionallantoic membrane assay

  • Stefania Pacini
  • Gabriele Morucci
  • Tiziana Punzi
  • Massimo Gulisano
  • Marco Ruggiero
Original article

Abstract

The effects of Gc protein-derived macrophage-activating factor (GcMAF) have been studied in cancer and other conditions where angiogenesis is deregulated. In this study, we demonstrate for the first time that the mitogenic response of human peripheral blood mononuclear cells (PBMCs) to GcMAF was associated with 3′-5′-cyclic adenosine monophosphate (cAMP) formation. The effect was dose dependent, and maximal stimulation was achieved using 0.1 ng/ml. Heparin inhibited the stimulatory effect of GcMAF on PBMCs. In addition, we demonstrate that GcMAF (1 ng/ml) inhibited prostaglandin E1- and human breast cancer cell-stimulated angiogenesis in chick embryo chorionallantoic membrane (CAM) assay. Finally, we tested different GcMAF preparations on CAM, and the assay proved to be a reliable, reproducible and inexpensive method to determine the relative potencies of different preparations and their stability; we observed that storage at room temperature for 15 days decreased GcMAF potency by about 50%. These data could prove useful for upcoming clinical trials on GcMAF.

Keywords

GcMAF cAMP Angiogenesis Peripheral blood human mononuclear cells 

Notes

Acknowledgments

This research project has been subsidized by the University of Firenze (Progetti di Ricerca di Ateneo, ex 60%) and by the Italian Ministry of Health (Progetto Strategico “La Medicina di genere come obiettivo strategico per la sanità pubblica: l’appropriatezza della cura per la tutela della salute della donna”). We are indebted with Prof. N. Yamamoto for providing GcMAF and inspiring discussion.

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Stefania Pacini
    • 1
  • Gabriele Morucci
    • 1
  • Tiziana Punzi
    • 1
  • Massimo Gulisano
    • 1
  • Marco Ruggiero
    • 2
  1. 1.Department of Anatomy, Histology and Forensic MedicineUniversity of FirenzeFlorenceItaly
  2. 2.Department of Experimental Pathology and OncologyUniversity of FirenzeFlorenceItaly

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