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Cancer Immunology, Immunotherapy

, Volume 59, Issue 6, pp 841–850 | Cite as

Circulating tumour-derived microvesicles in plasma of gastric cancer patients

  • Jaroslaw Baran
  • Monika Baj-Krzyworzeka
  • Kazimierz Weglarczyk
  • Rafal Szatanek
  • Maria Zembala
  • Jakub Barbasz
  • Antoni Czupryna
  • Antoni Szczepanik
  • Marek Zembala
Original Article

Abstract

Cell membrane microfragments called microvesicles (MV) originating from different cells are circulating in the blood of healthy subjects and their elevated numbers are found in different diseases, including cancer. This study was designed to characterise MV present in plasma of gastric cancer patients. Since majority of MV in blood are platelets-derived (PMV), plasma samples deprived of PMV were used. In comparison to control, the number of MV in patients was significantly elevated in all stages, higher in more advanced disease. Patients’ MV showed an increased membrane expression of CCR6 and HER-2/neu. The proportion of MV carrying some leucocyte determinants was low and similar in patients and control. Transmission electron microscopy showed their substantial heterogeneity in size and shape. The size determined by dynamic light scattering analysis confirmed this heterogeneity. The MV size distribution in patients was broader within the range of 10–800 nm, while in control MV showed 3-mode distribution within the range of 10–400 nm. Atomic force microscopy confirmed MV size heterogeneity with implication that larger objects represented aggregates of smaller microparticles. Patients’ MV exhibited increased absolute values of zeta potential, indicating a higher surface charge. Tumour markers HER-2/neu, MAGE-1, c-MET and EMMPRIN were detected both in control and patients’ samples with stronger expression in the latter. Significantly higher expression of MAGE-1 and HER-2/neu mRNA was observed in individual patients. All together, it suggests that at least some MV in plasma of gastric cancer patients are tumour-derived. However, their role in cancer requires further studies.

Keywords

Microvesicles Gastric cancer Chemokine receptors Tumour markers 

Abbreviations

AFM

Atomic force microscopy

MV

Microvesicles

PFP

Platelet-free plasma

PMV

Platelet-derived microvesicles

TEM

Transmission electron microscopy

TMV

Tumour-derived microvesicles

Notes

Acknowledgments

This study was supported by the State Committee for Scientific Research (grant no. 2 PO5A 049 29) and by Jagiellonian University Medical College (grant no: CRSW K/ZBW/000092). We thank Dr. Barbara Urbanowicz for TEM analysis and Mrs. Irena Ruggiero for skilful technical assistance.

Supplementary material

262_2009_808_MOESM1_ESM.pdf (484 kb)
Supplementary material 1 (PDF 484 kb)

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Jaroslaw Baran
    • 1
  • Monika Baj-Krzyworzeka
    • 1
  • Kazimierz Weglarczyk
    • 1
  • Rafal Szatanek
    • 1
  • Maria Zembala
    • 2
  • Jakub Barbasz
    • 2
    • 4
  • Antoni Czupryna
    • 3
  • Antoni Szczepanik
    • 3
  • Marek Zembala
    • 1
  1. 1.Department of Clinical Immunology, Polish-American Institute of PaediatricsJagiellonian University Medical CollegeCracowPoland
  2. 2.Institute of Catalysis and Surface ChemistryPolish Academy of SciencesCracowPoland
  3. 3.First Department of General and Gastrointestinal SurgeryJagiellonian University Medical CollegeCracowPoland
  4. 4.Institute of PhysicsJagiellonian UniversityCracowPoland

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