Cancer Immunology, Immunotherapy

, Volume 58, Issue 10, pp 1679–1690

Human CD80/IL2 lentivirus transduced acute myeloid leukaemia cells enhance cytolytic activity in vitro in spite of an increase in regulatory CD4+ T cells in a subset of cultures

  • Wendy Ingram
  • Shahram Kordasti
  • Lucas Chan
  • Linda D. Barber
  • Gee J. Tye
  • Nicola Hardwick
  • Ghulam J. Mufti
  • Farzin Farzaneh
Original Article

Abstract

Immunotherapeutic strategies are increasingly being explored as a method of enhancing anti-tumour immune responses in patients with acute myeloid leukaemia (AML). Regulatory CD4+ T cells (Tregs) suppress effector T and natural killer (NK) cells and therefore pose a potential challenge to the efficacy of immunotherapy. AML cells transduced with a lentivirus expressing CD80 (B7.1) and IL2 (LV-CD80/IL2) are capable of stimulating T and NK cell cytotoxicity in vitro. This study examines the effect of CD80/IL2 modified AML cells on Treg number and function. We report a significant increase in the number of CD8+ T cells (P = 0.046) CD3CD56+ NK cells (P = 0.028) and CD3+CD4+CD25highFoxp3+ Tregs (P = 0.043) following stimulation for 7 days with allogeneic LV-CD80/IL2 AMLs. In contrast, autologous LV-CD80/IL2 AML cell cultures provide a weaker stimulation with a lower number of CD8+ T cells (P = 0.011) and no change in NK cell or Treg numbers. However, an increase in cytotoxic CD8+ T cells and NK cells are detected following both allogeneic and autologous LV-CD80/IL2 stimulation as demonstrated by an increase in IFN-γ and CD107a expression. Despite the presence of increased numbers of Tregs with suppressive activity in a subset of cultures, increased lysis of unmodified AMLs was still achieved following allogeneic (day 0, 2.2%; day 7, 20.4%) and more importantly, autologous LV-CD80/IL2 culture in which AML patients had recently received intensive chemotherapy (day 0, 0%; day 7, 16%). Vaccination with LV-CD80/IL2 therefore provides a potential strategy to enhance anti-leukaemia immune responses without a concomitant stimulation of Treg-mediated inhibition of cytotoxic immunological responses.

Keywords

Regulatory T cells Acute myeloid leukaemia Lentivirus CD80 IL2 

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Wendy Ingram
    • 1
  • Shahram Kordasti
    • 1
  • Lucas Chan
    • 1
  • Linda D. Barber
    • 1
  • Gee J. Tye
    • 1
  • Nicola Hardwick
    • 1
  • Ghulam J. Mufti
    • 1
  • Farzin Farzaneh
    • 1
  1. 1.Department of Haematological MedicineKing’s College London, Rayne InstituteLondonUK

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