Cancer Immunology, Immunotherapy

, Volume 58, Issue 10, pp 1577–1586

Targeting tumor-associated macrophages in an experimental glioma model with a recombinant immunotoxin to folate receptor β

  • Taku Nagai
  • Masashi Tanaka
  • Yasuhiro Tsuneyoshi
  • Baohui Xu
  • Sara A. Michie
  • Kazuhisa Hasui
  • Hirofumi Hirano
  • Kazunori Arita
  • Takami Matsuyama
Original Article

DOI: 10.1007/s00262-009-0667-x

Cite this article as:
Nagai, T., Tanaka, M., Tsuneyoshi, Y. et al. Cancer Immunol Immunother (2009) 58: 1577. doi:10.1007/s00262-009-0667-x

Abstract

Tumor-associated macrophages (TAMs) are frequently found in glioblastomas and a high degree of macrophage infiltration is associated with a poor prognosis for glioblastoma patients. However, it is unclear whether TAMs in glioblastomas promote tumor growth. In this study, we found that folate receptor β (FRβ) was expressed on macrophages in human glioblastomas and a rat C6 glioma implanted subcutaneously in nude mice. To target FRβ-expressing TAMs, we produced a recombinant immunotoxin consisting of immunoglobulin heavy and light chain Fv portions of an anti-mouse FRβ monoclonal antibody and Pseudomonas exotoxin A. Injection of the immunotoxin into C6 glioma xenografts in nude mice significantly depleted TAMs and reduced tumor growth. The immunotoxin targeting FRβ-expressing macrophages will provide a therapeutic tool for human glioblastomas.

Keywords

Macrophage Glioma Immunotoxin Folate receptor beta 

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Taku Nagai
    • 1
  • Masashi Tanaka
    • 1
  • Yasuhiro Tsuneyoshi
    • 1
  • Baohui Xu
    • 2
  • Sara A. Michie
    • 2
  • Kazuhisa Hasui
    • 1
  • Hirofumi Hirano
    • 3
  • Kazunori Arita
    • 3
  • Takami Matsuyama
    • 1
  1. 1.Department of Immunology, Graduate School of Medical and Dental SciencesKagoshima UniversityKagoshimaJapan
  2. 2.Department of PathologyStanford University School of MedicineStanfordUSA
  3. 3.Department of Neurosurgery, Graduate School of Medical and Dental SciencesKagoshima UniversityKagoshimaJapan

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