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Cancer Immunology, Immunotherapy

, Volume 57, Issue 7, pp 1029–1038 | Cite as

Activation of cytotoxic T lymphocytes against CML28-bearing tumors by dendritic cells transduced with a recombinant adeno-associated virus encoding the CML28 gene

  • Li-Hua Xie
  • Fion Wan-Yee Sin
  • Samuel Chak-Sum Cheng
  • Ying-Kit Cheung
  • Kin-Tak Chan
  • Yi Xie
  • Yong Xie
Original Article

Abstract

Induction of anti-tumor immune responses by dendritic cells (DCs) transduced with a recombinant adeno-associated virus type 2 (rAAV2) encoding tumor antigens is considered a promising approach for cancer vaccine development. CML28, a novel antigen with the properties of cancer/testis (CT) antigens, is an attractive target for antigen-specific immunotherapy. Here we investigated the feasibility of inducing CML28-specific cytotoxic T lymphocyte (CTL) responses using DCs transduced with the rAAV2 vectors containing the CML28 gene (rAAV/CML28). Using an adenovirus-free packaging system, rAAV/CML28 was generated. The transduction efficiency of rAAV/CML28 in DCs increased in a multiplicity of infection (MOI)-dependent manner. The rAAV/CML28 transduction did not impair DC maturation, but even enhanced the CD80 expression. The rAAV/CML28-transduced DCs induced CML28-specific CTLs which exhibited a MHC class I-mediated antigen-specific lytic activity against CML28-bearing tumor cell lines (HepG2 and MCF-7) as well as the primary leukemia blasts. These findings suggest that rAAV/CML28-transduced DCs vaccine may serve as a feasible approach for the treatment of CML28-associated cancers.

Keywords

CML28 Adeno-associated virus Dendritic cell-based vaccine Cytotoxic T lymphocyte Cancer immunotherapy 

Notes

Acknowledgments

We express our gratitude to Professor Paul L. Hermonat and Dr. Yong Liu (University of Arkansas, Little Rock, AR, US) for their technical support.

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Li-Hua Xie
    • 1
    • 2
  • Fion Wan-Yee Sin
    • 1
  • Samuel Chak-Sum Cheng
    • 1
  • Ying-Kit Cheung
    • 1
  • Kin-Tak Chan
    • 1
  • Yi Xie
    • 2
  • Yong Xie
    • 1
  1. 1.Department of BiologyThe Hong Kong University of Science and TechnologyKowloonHong Kong, China
  2. 2.Department of Hematology, Huashan Hospital, the Medical CentreFudan UniversityShanghaiChina

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