Cancer Immunology, Immunotherapy

, Volume 56, Issue 12, pp 1945–1954

Specific CD8+ T cell responses to HLA-A2 restricted MAGE-A3 p271–279 peptide in hepatocellular carcinoma patients without vaccination

  • Hua-Gang Zhang
  • Hong-Song Chen
  • Ji-Run Peng
  • Xiao-Ying Shang
  • Jun Zhang
  • Qiao Xing
  • Xue-Wen Pang
  • Li-Ling Qin
  • Ran Fei
  • Ming-Hui Mei
  • Xi-Sheng Leng
  • Wei-Feng Chen
Original Article

Abstract

The MAGE-A3 protein, one of the promising tumor antigens for immunotherapy, is highly expressed in human hepatocellular carcinoma (HCC). In this study, we estimated the specific CD8+ T cell immune response to MAGE-A3 p271–279 peptide (M3271) in the peripheral blood of HCC patients without antigen vaccination in order to evaluate its immunotherapeutic potential in these patients. After expansion in vitro, the functional IFN-γ producing M3271 specific CD8+ T cells were detected in 30.8% (8/26) of HLA-A2+MAGE-A3+ HCC patients. The effector CD8+ T cells could release cytotoxic molecules of granzyme B and perforin after restimulation with natural HLA-A2+MAGE-A3+ HCC cell lines in the samples tested. The functional supertype of HLA-A2 in the presentation of HLA-A*0201 restricted M3271 peptide has been identified in the Chinese HCC patients of Han ethnicity, that widely expanded the applicability of this tumor peptide vaccine in Chinese HCC patients. Thus, the functionally detectable pre-existence of M3271-specific CD8+ T cells in HCC patients makes M3271 a potential target for immunotherapy in these patients. The responsive CD8+ T cells to both NY-ESO-1 and MAGE-A3 antigens provide a rationale for the application of a bivalent vaccine in HCC patients with tumors expressing both antigens.

Keywords

Antigens/Peptides/Epitopes Cytotoxic T cells Tumor immunity 

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Hua-Gang Zhang
    • 1
  • Hong-Song Chen
    • 2
  • Ji-Run Peng
    • 3
  • Xiao-Ying Shang
    • 1
  • Jun Zhang
    • 1
  • Qiao Xing
    • 1
  • Xue-Wen Pang
    • 1
  • Li-Ling Qin
    • 4
  • Ran Fei
    • 2
  • Ming-Hui Mei
    • 4
  • Xi-Sheng Leng
    • 3
  • Wei-Feng Chen
    • 1
  1. 1.Department of Immunology, School of Basic Medical SciencesPeking University Health Science CenterBeijingPeople’s Republic of China
  2. 2.Hepatology Institute, People’s HospitalPeking University Health Science CenterBeijingChina
  3. 3.Center of Hepatobiliary Surgery, People’s HospitalPeking University Health Science CenterBeijingChina
  4. 4.Guangxi Guilin Medical CollegeGuilinChina

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