Cancer Immunology, Immunotherapy

, Volume 56, Issue 7, pp 1087–1096

MIP3 alpha stimulates the migration of Langerhans cells in models of human papillomavirus (HPV)-associated (pre)neoplastic epithelium

  • Ludivine Herman
  • Pascale Hubert
  • Jean-Hubert Caberg
  • Brigitte Evrard
  • Witold Kedzia
  • Jacques Boniver
  • Philippe Delvenne
Original Article

DOI: 10.1007/s00262-006-0255-2

Cite this article as:
Herman, L., Hubert, P., Caberg, JH. et al. Cancer Immunol Immunother (2007) 56: 1087. doi:10.1007/s00262-006-0255-2

Abstract

Although human papillomavirus (HPV) DNA is detected in the majority of cervical cancers and their precursors (squamous intraepithelial lesions; SIL), the persistence or progression of cervical lesions could be associated with quantitative and functional alterations of dendritic/Langerhans cells (DC/LC). As LC abnormalities have been associated with a decreased expression of macrophage inflammatory protein 3α (MIP3α) in cervical SIL, we tested the effect of exogenous MIP3α on the migration of LC in a (pre)neoplastic epithelium formed in vitro. By using a Boyden chamber assay, we first showed that the migratory capacity of LC generated in vitro is significantly increased in the presence of MIP3α compared to control medium. We next demonstrated that MIP3α is able to increase the 3D infiltration of LC in organotypic cultures of HPV-transformed keratinocytes. This property to stimulate LC migration was not altered after inclusion of MIP3α in a bioadhesive polycarbophil gel. Moreover, the function of DC to exert cytostatic effects and to present alloantigens was not altered in the presence of MIP3α.

Keywords

Langerhans cells MIP3 alpha Human papillomavirus Uterine cervix Organotypic culture Cell migration 

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Ludivine Herman
    • 1
  • Pascale Hubert
    • 1
  • Jean-Hubert Caberg
    • 1
  • Brigitte Evrard
    • 2
  • Witold Kedzia
    • 3
  • Jacques Boniver
    • 1
  • Philippe Delvenne
    • 1
  1. 1.Department of Pathology, CRCE, B35University of LiegeLiegeBelgium
  2. 2.Department of Pharmaceutical Technology, CRCE, B35University of LiegeLiegeBelgium
  3. 3.Department of Gynecology and ObstetricsKarol Marcinkowski School of MedecinePoznanPoland

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