Cancer Immunology, Immunotherapy

, Volume 56, Issue 5, pp 709–717

HLA class I expression in metastatic melanoma correlates with tumor development during autologous vaccination

  • Teresa Cabrera
  • Ester Lara
  • José M. Romero
  • Isabel Maleno
  • Luis M. Real
  • Francisco Ruiz-Cabello
  • Pedro Valero
  • Francisco M. Camacho
  • Federico Garrido
Original Article

DOI: 10.1007/s00262-006-0226-7

Cite this article as:
Cabrera, T., Lara, E., Romero, J.M. et al. Cancer Immunol Immunother (2007) 56: 709. doi:10.1007/s00262-006-0226-7

Abstract

Our knowledge of the mechanisms underlying tumor-specific immune response and tumor escape has considerably increased. HLA class I antigen defects remain an important tumor escape mechanism since they influence the interactions between tumor cells and specific T and NK cells in the course of malignant disease. We have studied here HLA class I expression in six subcutaneous metastases obtained from a melanoma patient immunized with an autologous melanoma cell vaccine (M-VAX). We report in this paper that HLA class I antigen expression on these metastatic lesions strongly correlated with the course of the disease. The three metastases that were partially regressing at the time of their excision showed a strong HLA class I expression, whereas the progressing ones showed a very weak or negative staining with most of the anti-HLA class I mAbs used. Real-time quantitative PCR of the samples obtained from microdissected tumor tissue revealed a significant difference in the mRNA levels of HLA-ABC heavy chain and β2m between the two types of metastases, i.e., lower levels in progressing metastases and high levels in regressing ones, confirming the immunohistological findings. This is, to our knowledge, the first report where the clinical outcome of different HLA class I positive and negative melanoma metastases can be clearly correlated with the regression and progression of the disease, respectively.

Keywords

Melanoma HLA Metastasis Immunotherapy Cancer 

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Teresa Cabrera
    • 1
    • 2
  • Ester Lara
    • 1
    • 2
  • José M. Romero
    • 1
    • 2
  • Isabel Maleno
    • 1
  • Luis M. Real
    • 3
  • Francisco Ruiz-Cabello
    • 1
    • 2
  • Pedro Valero
    • 4
  • Francisco M. Camacho
    • 5
  • Federico Garrido
    • 1
    • 2
  1. 1.Servicio de Análisis ClínicosHospital Universitario Virgen de las NievesGranadaSpain
  2. 2.Departamento de Bioquímica, Biología Molecular 3 e Inmunología, Facultad de MedicinaUniversidad de GranadaGranadaSpain
  3. 3.Departamento de Genómica EstructuralNeocodexSevillaSpain
  4. 4.Clinica Infanta LuisaSevillaSpain
  5. 5.Departamento de Dermatología Médico-Quirúrgica y Venereología, Hospital Universitario “Virgen Macarena”Universidad de SevillaSevillaSpain

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