Cancer Immunology, Immunotherapy

, Volume 55, Issue 7, pp 808–818 | Cite as

Tumour-derived microvesicles carry several surface determinants and mRNA of tumour cells and transfer some of these determinants to monocytes

  • Monika Baj-Krzyworzeka
  • Rafał Szatanek
  • Kazimierz Węglarczyk
  • Jarosław Baran
  • Barbara Urbanowicz
  • Piotr Brański
  • Mariusz Z. Ratajczak
  • Marek Zembala
Original Article

Abstract

This study was designed to determine the characteristics of tumour cell-derived microvesicles (TMV) and their interactions with human monocytes. TMV were shed spontaneously by three different human cancer cell lines but their release was significantly increased upon activation of the cells with phorbol 12-myristate 13-acetate (PMA). TMV showed the presence of several surface determinants of tumour cells, e.g. HLA class I, CD29, CD44v7/8, CD51, chemokine receptors (CCR6, CX3CR1), extracellular matrix metalloproteinase inducer (EMMPRIN), epithelial cell adhesion molecule (EpCAM), but their level of expression differed from that on cells they originated from. TMV also carried mRNA for growth factors: vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), interleukin-8 (IL-8) and surface determinants (CD44H). TMV were localized at the monocytes surface following their short exposure to TMV, while at later times intracellularly. TMV transferred CCR6 and CD44v7/8 to monocytes, exerted antiapoptotic effect on monocytes and activated AKT kinase (Protein Kinase B). Thus, TMV interact with monocytes, alter their immunophenotype and biological activity. This implicates the novel mechanism by which tumour infiltrating macrophages may be affected by tumour cells not only by a direct cell to cell contact, soluble factors but also by TMV.

Keywords

Tumour-derived microvesicles Monocytes Transfer CCR6 mRNA 

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Monika Baj-Krzyworzeka
    • 1
  • Rafał Szatanek
    • 1
  • Kazimierz Węglarczyk
    • 1
  • Jarosław Baran
    • 1
  • Barbara Urbanowicz
    • 3
  • Piotr Brański
    • 4
  • Mariusz Z. Ratajczak
    • 2
  • Marek Zembala
    • 1
  1. 1.Department of Clinical ImmunologyPolish-American Institute of Paediatrics, Jagiellonian University Medical CollegeCracowPoland
  2. 2.Department of TransplantologyPolish-American Institute of Paediatrics, Jagiellonian University Medical CollegeCracowPoland
  3. 3.Department of PathologyPolish-American Institute of Paediatrics, Jagiellonian University Medical CollegeCracowPoland
  4. 4.Department of Neurobiology, Institute of PharmacologyPolish Academy of ScienceCracowPoland

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