Cancer Immunology, Immunotherapy

, Volume 55, Issue 6, pp 644–652 | Cite as

A new LAGE-1 peptide recognized by cytolytic T lymphocytes on HLA-A68 tumors

  • Zhaojun Sun
  • Bernard Lethé
  • Yi Zhang
  • Vincenzo Russo
  • Didier Colau
  • Vincent Stroobant
  • Thierry Boon
  • Pierre van der BruggenEmail author
Original Article


Antigens encoded by genes of the LAGE family, including LAGE-1 and NY-ESO-1, are of interest for cancer immunotherapy because they are tumor-specific and shared by tumors of different histological types. Several clinical trials are in progress with NY-ESO-1 peptides, protein, recombinant poxviruses, and dendritic cells pulsed with peptides. In this study, CD8 T lymphocytes from an individual without cancer were stimulated with dendritic cells infected with a recombinant avian poxvirus encoding a complete LAGE-1 protein. A CTL clone was isolated that recognized a new LAGE-1 peptide, ELVRRILSR, which corresponds to position 103–111 of the protein sequence. It is presented by HLA-A6801 molecules. When tumor cells expressing LAGE-1 were transfected with HLA-A68, they were lysed by the CTL clone, indicating that the peptide is processed in tumor cells. These results indicate that the LAGE-1.A68 peptide can be used for antitumoral vaccination. We observed also that specific T cells could be detected in a blood sample with a high sensitivity by using an A68/LAGE-1 fluorescent multimer.


HLA-A68 LAGE-1 Peptide Poxvirus SNP Tumor 



We thank Dr. Pierre Coulie for the critical reading and Mrs Nathalie Krack for the editorial assistance. This work was supported by the Belgian Programme on Interuniversity Poles of Attraction initiated by the Belgian State, Prime Minister’s Office, Science Policy Programming, and by a grant from the Fédération Belge contre le Cancer (Belgium).


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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Zhaojun Sun
    • 1
  • Bernard Lethé
    • 1
  • Yi Zhang
    • 1
    • 2
  • Vincenzo Russo
    • 3
  • Didier Colau
    • 1
  • Vincent Stroobant
    • 1
  • Thierry Boon
    • 1
  • Pierre van der Bruggen
    • 1
    Email author
  1. 1.Ludwig Institute for Cancer Research Institute of Cellular Pathology, Cellular Genetics UnitUniversité de LouvainBrusselsBelgium
  2. 2.Department of PathologyUniversity of ChicagoChicagoUSA
  3. 3.Cancer Immunotherapy and Gene Therapy ProgramScientific Institute H. San RaffaeleMilanoItaly

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