Cancer Immunology, Immunotherapy

, Volume 52, Issue 10, pp 643–647

Generation of non-permissive basement membranes by anti-laminin antibody fragments produced by matrix-embedded gene-modified cells

  • Laura Sanz
  • Mónica Feijóo
  • Belén Blanco
  • Antonio Serrano
  • Luis Álvarez-Vallina
Original Article

Abstract

Tumor-induced blood vessel formation is a key process for the growth and spread of solid tumors, traditionally attributed to activated host endothelial cells (angiogenesis). Recently, highly aggressive cancer cells have been shown to form vascular channels in the absence of endothelial cells (vasculogenic mimicry). In this work, we have focused on the common dependence of both processes in their interactions with the surrounding extracellular matrix. We had previously described a human recombinant anti-laminin antibody that blocked the capillary morphogenesis of human endothelial cells. Here, we demonstrate that the purified antibody is capable of inhibiting channel formation by human cancer cells, suggesting a common morphogenic pathway in both processes. Moreover, matrix-embedded cells producing antibody fragments may render the surrounding matrix non-permissive for aggressive tumor cells. These results open the way for the development of new therapeutic strategies for cancer.

Keywords

Extracellular matrix Laminin Capillary morphogenesis Tumor plasticity Single-chain antibody fragments 

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Laura Sanz
    • 1
  • Mónica Feijóo
    • 1
  • Belén Blanco
    • 1
  • Antonio Serrano
    • 2
  • Luis Álvarez-Vallina
    • 1
  1. 1.Department of ImmunologyHospital Universitario Clínica Puerta de HierroMadridSpain
  2. 2.Department of Immunology and OncologyCentro Nacional de BiotecnologíaMadridSpain

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