Cancer Immunology, Immunotherapy

, Volume 52, Issue 8, pp 506–512 | Cite as

Targeted cytotoxic effect of anti-JL1 immunotoxin against a human leukemic cell line and its clinical implications

  • Young Kee Shin
  • Yoon La Choi
  • Eun Young Choi
  • Min Kyung Kim
  • Myeong-Cherl Kook
  • Junho Chung
  • Yang-Kyu Choi
  • Heung Sik Kim
  • Hyung Geun Song
  • Seong Hoe Park
Original Article

Abstract

We have previously reported the identification of a unique thymocyte-specific surface molecule, JL1, which was detected using the monoclonal antibody (mAb), anti-JL1. Interestingly, JL1 was shown to be expressed in most leukemias, irrespective of their immunophenotype, and subpopulations of normal bone marrow (BM) mononuclear cells (MNCs). Here we investigated the potential usefulness of the anti-JL1 mAb as a therapeutic tool for leukemia. We demonstrated that the proliferation of cultured human leukemia cells was dramatically inhibited in vitro by anti-JL1 mAb conjugated with the polypeptide toxin, gelonin, but not by gelonin alone. We then systematically investigated the reactivity of the anti-JL1 mAb against normal human tissues to evaluate possible side effects along with various hematopoietic and nonhematopoietic tumor cell lines. All of 33 types of normal tissues except thymus and subpopulation of BM MNCs were clearly devoid of JL1 expression. Among tumor cell lines, all the nonhematopoietic cell lines tested were negative for JL1 expression, while some hematopoietic cell lines contained JL1 antigen. Collectively, the results showed the cytotoxic effects of anti-JL1-based immunotoxin against JL1-positive leukemic cells, sparing most normal tissues other than thymocytes and some BM MNCs. Therefore, we strongly suggest that gelonin-conjugated anti-JL1 mAb immunotoxin could be developed as a potential immunotherapeutic agent in the treatment of various types of JL1-positive acute leukemias.

Keywords

JL1 Leukemia Immunotoxin Gelonin 

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Young Kee Shin
    • 1
    • 8
  • Yoon La Choi
    • 1
  • Eun Young Choi
    • 1
  • Min Kyung Kim
    • 2
  • Myeong-Cherl Kook
    • 1
  • Junho Chung
    • 3
    • 4
  • Yang-Kyu Choi
    • 5
  • Heung Sik Kim
    • 6
  • Hyung Geun Song
    • 7
  • Seong Hoe Park
    • 1
  1. 1.Department of PathologySeoul National University College of MedicineSeoulKorea
  2. 2.Department of Pathology, Ilsan Paik HospitalInje University College of MedicineIlsanKorea
  3. 3.Department of Biochemistry and Molecular Biology, Cancer Research InstituteSeoul National University College of MedicineSeoulKorea
  4. 4.National Cancer CenterGoyangKorea
  5. 5.Genetic Resources CenterKorea Research Institute of Bioscience and BiotechnologyDaejeonKorea
  6. 6.Department of PediatricsKeimyung University College of MedicineTaeguKorea
  7. 7.Department of PathologyChungbuk National University College of MedicineChungjuKorea
  8. 8.DiNonA Inc.SeoulKorea

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