Cancer Immunology, Immunotherapy

, Volume 51, Issue 11–12, pp 669–673 | Cite as

Mucin gene (MUC1) transfected dendritic cells as vaccine: results of a phase I/II clinical trial

  • Gabriele Pecher
  • Arnt Häring
  • Lothar Kaiser
  • Eckhard Thiel
Original Article

Abstract.

Dendritic cells (DC) derived from peripheral blood monocytes are currently being investigated in clinical trials for their role in stimulating the immune system. We performed a phase I/II clinical trial using human autologous DC transfected with cDNA of the human tumor antigen mucin (MUC1) as a vaccine in 10 patients with advanced breast, pancreatic or papillary cancer. After liposomal transfection, flow cytometry testing showed that 2% to 53% of the DC expressed mucin epitopes. Patients were immunized two or three times with 1 million transfected DC injected subcutaneously (s.c.). A vaccine-specific delayed-type hypersensitivity (DTH) reaction was observed in 3 out of 10 patients. After vaccination, 4 patients showed a 2- to 10-fold increase in the frequency of mucin-specific interferon-gamma (IFN-γ)-secreting CD8+ T cells. We demonstrated the feasibility and safety of a vaccine consisting of autologous gene-transfected DC, and that immunologic responses could be induced even in patients with pretreated and advanced disease.

Dendritic cell Gene transfer Immunotherapy Mucin (MUC1) Vaccine 

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Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • Gabriele Pecher
    • 1
  • Arnt Häring
    • 1
  • Lothar Kaiser
    • 2
  • Eckhard Thiel
    • 1
  1. 1.Department of Hematology and Oncology, Free University of Berlin, Universitätsklinikum Benjamin Franklin, Berlin, Germany
  2. 2.Immunological Laboratory Hannover, Hannover, Germany
  3. 3.Max-Delbrück Center for Molecular Medicine, Berlin, Germany
  4. 4.Current address: Medical Clinic for Oncology and Hematology, Charité Campus Mitte, Humboldt University Berlin, Schumannstr. 20/21, 10117 Berlin, Germany

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