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Cancer Immunology, Immunotherapy

, Volume 50, Issue 12, pp 682–690 | Cite as

SV40 Tag-specific cytotoxic T lymphocytes generated from the peripheral blood of malignant pleural mesothelioma patients

  • Robert K. Bright
  • Eric T. Kimchi
  • Michael H. Shearer
  • Ronald C. Kennedy
  • Harvey I. Pass
Original Article

Abstract.

Malignant pleural mesothelioma (MPM) is an aggressive cancer, with survival of less than one year following diagnosis and treatment with current protocols. Recent studies have demonstrated the presence of the simian virus 40 (SV40)-like, large tumor antigen (Tag) in nearly 60% of MPMs. SV40 Tag is a viral-encoded tumor-specific antigen, and thus a potential target for the induction of anti-tumor immunity and the development of therapeutic vaccines. We describe here evidence for the existence of SV40 Tag-specific immune responses in patients with MPM whose tumors express Tag. Humoral immunity was demonstrated by the detection of IgG titers against Tag in serum samples from 1/3 of patients examined. CTLs were generated from the peripheral blood of an HLA-A2+ MPM patient with a synthetic peptide representing an HLA-A2 binding epitope in SV40 Tag. The CTLs demonstrated epitope fine specificity, in that other peptides from SV40 Tag and a peptide from influenza virus were not recognized in the context of HLA-A2. Moreover, the CTLs were capable of recognizing mesothelioma tumor cells that expressed SV40 Tag, in an MHC class I restricted manner.

SV40 Tag CTLs Peptides Mesothelioma Tumor 

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Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • Robert K. Bright
    • 1
  • Eric T. Kimchi
    • 2
  • Michael H. Shearer
    • 3
  • Ronald C. Kennedy
    • 3
  • Harvey I. Pass
    • 2
  1. 1.Laboratory of Prostate Cancer Biology, Robert W. Franz Cancer Research Center, Earle A. Chiles Research Institute, and the Oregon Cancer Center, 4805 NE Glisan Street, Portland, OR 97213, USA
  2. 2.Karmanos Cancer Institute and the Department of Surgery, Wayne State University School of Medicine, Detroit, MI 48201, USA
  3. 3.Department of Microbiology and Immunology, Texas Tech University, Lubbock, TX 79430, USA

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