Measurement of central µ-opioid receptor binding in vivo with PET and [11C]carfentanil: a test–retest study in healthy subjects
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[11C]Carfentanil has been widely used in positron emission tomography (PET) studies for measuring µ-opioid receptor binding in humans, but the reproducibility of the binding parameter estimates is unknown.
Materials and methods
Eight healthy volunteers were scanned twice during the same day with [11C]carfentanil PET, and binding to receptors was assessed with both reference tissue and arterial plasma input-based models using region of interest (ROI) and voxel-based quantification.
The two-tissue compartmental model distribution volume (VT) was highly reproducible as indicated by low variability (VAR < 6%) and high intraclass correlation coefficients (ICC > 0.93). BPND (BP relative to the nondisplaceable tissue compartment) was also highly reproducible (VAR < 10%, ICC > 0.90) both at ROI- and voxel-level, and reference tissue-based models provided stable estimates after 40 min.
The reproducibility of [11C]carfentanil binding parameter estimates is excellent with outcome measures based on both arterial plasma and reference tissue input, and a scanning time of 40 min appears sufficient.
Keywords(11C)carfentanil Mu opioid receptor Positron emission tomography Imaging
The staffs of the MRI and PET units at Turku PET Centre are gratefully appreciated.
Disclosures/Conflicts of Interest
The authors declare no conflicts of interest.
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