Biodisposition and metabolism of [18F]fluorocholine in 9L glioma cells and 9L glioma-bearing fisher rats

  • Aditya Bansal
  • Wang Shuyan
  • Toshiko Hara
  • Robert A. Harris
  • Timothy R. DeGrado
Original Article

Abstract

Purpose

[18F]Fluorocholine ([18F]FCH) was developed as an analog of [11C]choline for tumor imaging; however, its metabolic handling remains ill defined. In this study, the metabolism of [18F]FCH is evaluated in cultured 9L glioma cells and Fisher 344 rats bearing 9L glioma tumors.

Methods

9L glioma cells were incubated with [18F]FCH and [14C]choline under normoxic and hypoxic (1% O2) conditions and analyzed for metabolic fate. [18F]FCH and [14C]choline kinetics and metabolism were studied in Fisher 344 rats bearing subcutaneous 9L tumors.

Results

[18F]FCH and [14C]choline were similarly metabolized in 9L cells in both normoxic and hypoxic conditions over a 2-h incubation period. In normoxia, radioactivity was predominantly in phosphorylated form for both tracers after 5-min incubation. In hypoxia, the tracers remained mainly in nonmetabolized form at early timepoints (<20 min). Slow dephosphorylation of intracellular [18F]phosphofluorocholine (0.043–0.060 min−1) and [14C]phosphocholine (0.072–0.088 min−1) was evidenced via efflux measurements. In rat, both [18F]FCH and [14C]choline showed high renal and hepatic uptake. Blood clearance of both tracers was rapid with oxidative metabolites, [18F]fluorobetaine and [14C]betaine, representing the majority of radiolabel in plasma after 5 min postinjection. Oxidation (in liver) and lipid incorporation (in lung) were somewhat slower for [18F]FCH relative to [14C]choline. The majority of radiolabel in hypoxic subcutaneous tumor, as in hypoxic cultured 9L cells, was found as nonmetabolized [18F]FCH and [14C]choline.

Conclusions

[18F]FCH mimics choline uptake and metabolism by 9L glioma cells and tumors. However, subtle changes in biodistribution, oxidative metabolism, dephosphorylation, lipid incorporation, and renal excretion show moderate effects of the presence of the radiofluorine atom in [18F]FCH. The decrease in phosphorylation of exogenous choline by cancer cells should be considered in interpretation of positron emission tomography images in characteristically hypoxic tumors.

Keywords

Choline Fluorocholine 189L glioma Rat Metabolism Hypoxia 

Notes

Acknowledgement

The work was funded in part by National Institutes of Health (RO1 CA108620, R01 HL-63371) and the Indiana Genomics Initiative Program of Indiana University School of Medicine (IUSM), a grant from the Lilly Endowment. The authors thank Dr. Frank A. Witzmann and Seokmin Hong in Department of Cellular and Integrative Physiology, IUSM for their help in liquid chromatography–mass spectrometry quantification of [19F]FCH.

Conflict of interest statement

No authors have affiliations that present conflicts of interest for this work.

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Aditya Bansal
    • 1
  • Wang Shuyan
    • 2
  • Toshiko Hara
    • 2
  • Robert A. Harris
    • 1
  • Timothy R. DeGrado
    • 1
    • 2
  1. 1.Department of Biochemistry and Molecular BiologyIndiana University School of MedicineIndianapolisUSA
  2. 2.Department of RadiologyIndiana University School of MedicineIndianapolisUSA

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