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In vivo calcium imaging of the aging and diseased brain

  • Gerhard Eichhoff
  • Marc Aurel Busche
  • Olga GaraschukEmail author
Article

Abstract

Purpose

Over the last decade, in vivo calcium imaging became a powerful tool for studying brain function. With the use of two-photon microscopy and modern labelling techniques, it allows functional studies of individual living cells, their processes and their interactions within neuronal networks. In vivo calcium imaging is even more important for studying the aged brain, which is hard to investigate in situ due to the fragility of neuronal tissue.

Methods

In this article, we give a brief overview of the techniques applicable to image aged rodent brain at cellular resolution.

Results

We use multicolor imaging to visualize specific cell types (neurons, astrocytes, microglia) as well as the autofluorescence of the “aging pigment” lipofuscin.

Conclusions

Further, we illustrate an approach for simultaneous imaging of cortical cells and senile plaques in mouse models of Alzheimer’s disease.

Keywords

In vivo calcium imaging Plaques Alzheimer’s disease Lipofuscin 

Notes

Acknowledgements

This work is supported by grants of the Deutsche Forschungsgemeinschaft (SFB 391 and SFB 596) and the Bundesministerium für Bildung und Forschung (NGFN-2). We are thankful to M. Staufenbiel (Novartis Pharma, Basel, Switzerland) for AD mouse mutants, M. Kerschensteiner and T. Misgeld for CX3CR1-EGFP mice and A. Konnerth for support and helpful discussions. We thank Olympus Europa for providing two-photon-based Fluoview 1000MPE.

Conflict of interest statement

The authors declare that they have no relevant financial or any other interests in this manuscript.

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Gerhard Eichhoff
    • 1
  • Marc Aurel Busche
    • 1
  • Olga Garaschuk
    • 1
    Email author
  1. 1.Institute of NeuroscienceTechnical University of MunichMunichGermany

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