Effects of anesthetic agents on cellular 123I-MIBG transport and in vivo 123I-MIBG biodistribution

  • Bong-Ho Ko
  • Jin-Young Paik
  • Kyung-Ho Jung
  • Jun-Sang Bae
  • Eun Jung Lee
  • Yearn Seong Choe
  • Byung-Tae Kim
  • Kyung-Han Lee
Original article

Abstract

Objectives

Small animal imaging with meta-iodobenzylguanidine (MIBG) allows characterization of animal models, optimization of tumor treatment strategies, and monitoring of gene expression. Anesthetic agents, however, can affect norepinephrine (NE) transport and systemic sympathetic activity. We thus elucidated the effects of anesthetic agents on MIBG transport and biodistribution.

Methods

SK-N-SH neuroblastoma and PC-12 pheochromocytoma cells were measured for 123I-MIBG uptake after treatment with ketamine (Ke), xylazine (Xy), Ke/Xy, or pentobarbital (Pb). NE transporters were assessed by Western blots. Normal ICR mice and PC-12 tumor-bearing mice were injected with 123I-MIBG 10 min after anesthesia with Ke/Xy, Ke, Xy, or Pb. Plasma NE levels and MIBG biodistribution were assessed.

Results

Cellular 123I-MIBG uptake was dose-dependently inhibited by Ke and Xy but not by Pb. Treatment for 2 h with 300 μM Ke, Xy, and Ke/Xy decreased uptake to 46.0 ± 1.6, 24.8 ± 1.5, and 18.3 ± 1.6% of controls. This effect was completely reversed by fresh media, and there was no change in NE transporter levels. In contrast, mice anesthetized with Ke/Xy showed no decrease of MIBG uptake in target organs. Instead, uptakes and organ-to-blood ratios were increased in the heart, lung, liver, and adrenals. Plasma NE was notably reduced in the animals with corresponding decreases in blood MIBG, which partly contributed to the increase in target organ uptake.

Conclusion

In spite of their inhibitory effect at the transporter level, Ke/Xy anesthesia is a satisfactory method for MIBG imaging that allows favorable target tissue uptake and contrast by reducing circulating NE and MIBG.

Keywords

Anesthesia 123I-MIBG Tumor Norepinephrine Transporter Myocardium 

Notes

Acknowledgments

This work was supported by the Korea Research Foundation Grant KRF-2005-202-E00116.

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Bong-Ho Ko
    • 1
  • Jin-Young Paik
    • 1
  • Kyung-Ho Jung
    • 1
  • Jun-Sang Bae
    • 1
  • Eun Jung Lee
    • 1
  • Yearn Seong Choe
    • 1
  • Byung-Tae Kim
    • 1
  • Kyung-Han Lee
    • 1
  1. 1.Department of Nuclear Medicine, Samsung Medical CenterSungkyunkwan University School of MedicineSeoulSouth Korea

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