In vivo radionuclide uptake quantification using a multi-pinhole SPECT system to predict renal function in small animals
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In vivo quantification of radiopharmaceuticals has great potential as a tool in developing new drugs. We investigated the accuracy of in vivo quantification with multi-pinhole single-photon emission computed tomography (SPECT) in rats.
Fifteen male Lewis rats with different stages of renal dysfunction were injected with 50 MBq 99mTc-dimercaptosuccinic acid. Four to six hours after injection, SPECT of the kidneys was acquired with a new four-headed multi-pinhole collimator camera. Immediately after imaging the rats were sacrificed and the kidneys were counted in a gamma-counter to determine the absorbed activity. SPECT data were reconstructed iteratively and regions of interest (ROIs) were drawn manually. The absolute activity in the ROIs was determined.
Uptake values ranging from 0.71% to 21.87% of the injected activity were measured. A very strong linear correlation was found between the determined activity in vivo and ex vivo (r 2=0.946; slope m=1.059).
Quantification in vivo using this multi-pinhole SPECT system is highly accurate.
KeywordsAnimal SPECT In vivo quantification 99mTc-DMSA Renal uptake
Support was provided by the Swiss National Science Foundation, the Novartis Foundation (Switzerland), the Dutch Organisation for Scientific Research (ZonMw) (the Netherlands) and the Alexander von Humboldt Foundation. The authors wish to thank all supporting personnel of the Department of Nuclear Medicine, Erasmus MC, Rotterdam, and especially Marleen Melis, for their expert help and effort.
- 5.Kabasakal L, Turkmen C, Ozmen O, Alan N, Onsel C, Uslu I. Is furosemide administration effective in improving the accuracy of determination of differential renal function by means of technetium-99m DMSA in patients with hydronephrosis. Eur J Nucl Med Mol Imaging 2002;29:1433–1437PubMedCrossRefGoogle Scholar