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18F-choline in experimental soft tissue infection assessed with autoradiography and high-resolution PET

  • Matthias T. Wyss
  • Bruno Weber
  • Michael Honer
  • Nicolas Späth
  • Simon M. Ametamey
  • Gerrit Westera
  • Beata Bode
  • Achim H. Kaim
  • Alfred BuckEmail author
Original Article

Abstract

For each oncological tracer it is important to know the uptake in non-tumorous lesions. The purpose of this study was to measure the accumulation of fluorine-18 choline (FCH), a promising agent for the evaluation of certain tumour types, in infectious tissue. Unilateral thigh muscle abscesses were induced in five rats by intramuscular injection of 0.1 ml of a bacterial suspension (Staphylococcus aureus, 1.2×109 CFU/ml). In all animals, FCH accumulation was measured with high-resolution positron emission tomography (PET) on day 6. Autoradiography of the abscess and ipsilateral healthy muscle was performed on day 7 (three animals) and day 11 (two animals) and correlated with histology. In addition, 18F-fluorodeoxyglucose (FDG) PET was performed on day 5. Increased FCH uptake was noted in specific layers of the abscess wall which contained an infiltrate of mainly granulocytes on day 7 and mainly macrophages on day 11. The autoradiographic standardised uptake values in the most active part of the abscess wall were 2.99 on day 7 (n=3) and 4.05 on day 11 (n=2). In healthy muscle the corresponding values were 0.99 and 0.64. The abscesses were clearly visualised on the FCH and FDG PET images. In conclusion, this study demonstrated avid FCH accumulation in inflammatory tissue, which limits the specificity of FCH for tumour detection. Future studies are now needed to determine the degree of this limitation in human cancer patients.

Keywords

Soft tissue infection 18F-choline 18F-fluorodeoxyglucose Autoradiography PET Tumour imaging 

Notes

Acknowledgements

The authors would like to thank Claudia Keller for the help with the data acquisition and Tibor Cservenjak for the radiotracer synthesis. This project was supported by the Wilhelm Sander Foundation.

References

  1. 1.
    Kaim AH, Weber B, Kurrer MO, Westera G, Schweitzer A, Gottschalk J, von Schulthess GK, Buck A.18F-FDG and 18F-FET uptake in experimental soft tissue infection. Eur J Nucl Med Mol Imaging 2002; 29:648–654.PubMedGoogle Scholar
  2. 2.
    Weber WA, Wester HJ, Grosu AL, Herz M, Dzewas B, Feldmann HJ, Molls M, Stocklin G, Schwaiger M. O-(2-[18F]fluoroethyl)-l-tyrosine and l-[methyl-11C]methionine uptake in brain tumours: initial results of a comparative study. Eur J Nucl Med 2000; 27:542–549.PubMedGoogle Scholar
  3. 3.
    Hustinx R, Lemaire C, Jerusalem G, Moreau P, Cataldo D, Duysinx B, Aerts J, Fassotte MF, Foidart J, Luxen A. Whole-body tumor imaging using PET and 2-18F-fluoro-L-tyrosine: preliminary evaluation and comparison with 18F-FDG. J Nucl Med 2003; 44:533–539.PubMedGoogle Scholar
  4. 4.
    Hara T, Kosaka N, Kishi H. PET imaging of prostate cancer using carbon-11-choline. J Nucl Med 1998; 39:990–995.PubMedGoogle Scholar
  5. 5.
    de Jong IJ, Pruim J, Elsinga PH, Vaalburg W, Mensink HJ. Preoperative staging of pelvic lymph nodes in prostate cancer by11C-choline PET. J Nucl Med 2003; 44:331–335.PubMedGoogle Scholar
  6. 6.
    de Jong IJ, Pruim J, Elsinga PH, Vaalburg W, Mensink HJ, Visualization of prostate cancer with11C-choline positron emission tomography. Eur Urol 2002; 42:18–23.CrossRefPubMedGoogle Scholar
  7. 7.
    DeGrado TR, Baldwin SW, Wang S, Orr MD, Liao RP, Friedman HS, Reiman R, Price DT, Coleman RE. Synthesis and evaluation of (18)F-labeled choline analogs as oncologic PET tracers. J Nucl Med 2001; 42:1805–1814.PubMedGoogle Scholar
  8. 8.
    DeGrado TR, Coleman RE, Wang S, Baldwin SW, Orr MD, Robertson CN, Polascik TJ, Price DT. Synthesis and evaluation of18F-labeled choline as an oncologic tracer for positron emission tomography: initial findings in prostate cancer. Cancer Res 2001; 61:110–117.PubMedGoogle Scholar
  9. 9.
    DeGrado TR, Reiman RE, Price DT, Wang S, Coleman RE. Pharmacokinetics and radiation dosimetry of18F-fluorocholine. J Nucl Med 2002; 43:92–96.PubMedGoogle Scholar
  10. 10.
    Price DT, Coleman RE, Liao RP, Robertson CN, Polascik TJ, DeGrado TR. Comparison of [18F]fluorocholine and [18F]fluorodeoxyglucose for positron emission tomography of androgen dependent and androgen independent prostate cancer. J Urol 2002; 168:273–280.PubMedGoogle Scholar
  11. 11.
    Mikolajczyk K, Szabatin M, Rudnicki P, Grodzki M, Burger C. A JAVA environment for medical image data analysis: initial application for brain PET quantitation. Med Inform (Lond) 1998; 23:207–214.Google Scholar
  12. 12.
    Kaim AH, Weber B, Kurrer M, Gottschalk J, von Schulthess GK, Buck A. Autoradiographic quantification of18F-FDG uptake in experimental soft tissue abscesses in rats. Radiology 2002; 223:446–451.PubMedGoogle Scholar
  13. 13.
    Zhang H, Tian M, Oriuchi N, Higuchi T, Watanabe H, Aoki J, Tanada S, Endo K.11C-choline PET for the detection of bone and soft tissue tumours in comparison with FDG PET. Nucl Med Commun 2003; 24:273–279.CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Matthias T. Wyss
    • 1
    • 2
  • Bruno Weber
    • 1
  • Michael Honer
    • 2
  • Nicolas Späth
    • 1
  • Simon M. Ametamey
    • 2
  • Gerrit Westera
    • 2
  • Beata Bode
    • 4
  • Achim H. Kaim
    • 3
  • Alfred Buck
    • 1
    Email author
  1. 1.PET Center, Division of Nuclear MedicineUniversity Hospital ZurichZurichSwitzerland
  2. 2.Center for Radiopharmaceutical Science of ETH, PSI and USZPaul Scherrer InstituteVilligenSwitzerland
  3. 3.Klinik Im SchachenAarauSwitzerland
  4. 4.Institute of PathologyUniversity HospitalZurichSwitzerland

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