Advertisement

18F-FDG and 18F-FET uptake in experimental soft tissue infection

  • Achim H. Kaim
  • Bruno Weber
  • Michael O. Kurrer
  • Gerrit Westera
  • Alain Schweitzer
  • Jochen Gottschalk
  • Gustav K. von Schulthess
  • Alfred Buck
Original Article

Abstract.

The aim of this study was to compare the uptake of 18F-fluoroethyl-L-tyrosine (18F-FET) with that of 18F-fluorodeoxyglucose (18F-FDG) in activated inflammatory white blood cells. Unilateral thigh muscle abscesses were induced in 11 rats by intramuscular inoculation of 0.1 ml of a bacterial suspension (S. aureus, 1.2×109 CFU/ml). Four animals were intraperitoneally injected with 130–180 MBq 18F-FDG, four with 140–170 MBq 18F-FET and three with a mixture of 140–170 MBq 18F-FET and 1.8 MBq 14C-deoxyglucose. Autoradiography (10 µm slice thickness) of the abscess and the contralateral muscle was performed and detailed spatial correlation of autoradiography and histopathology (haematoxylin-eosin staining) was obtained. Regions of interest were placed on the abscess wall and the grey values (digitised image intensities) measured were converted to kBq/cc per kBq injected activity per gram (SUV). Areas with increased 18F-FDG uptake corresponded to cellular inflammatory infiltrates mainly consisting of granulocytes. The SUV was calculated to be 4.08±0.65 (mean±SD). The uptake of 18F-FET in activated white blood cells was not increased: the SUV of the abscess wall, at 0.74±0.14, was even below that of contralateral muscle. The low uptake of 18F-FET in non-neoplastic inflammatory cells promises a higher specificity for the detection of tumour cells than is achieved with 18F-FDG, since the immunological host response will not be labelled and inflammation can be excluded.

Soft tissue infection Fluorine-18 fluorodeoxyglucose Autoradiography Fluorine-18 fluoroethyl-L-tyrosine 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • Achim H. Kaim
    • 1
  • Bruno Weber
    • 1
  • Michael O. Kurrer
    • 2
  • Gerrit Westera
    • 3
  • Alain Schweitzer
    • 4
  • Jochen Gottschalk
    • 5
  • Gustav K. von Schulthess
    • 1
  • Alfred Buck
    • 1
  1. 1.Nuclear Medicine, University Hospital Zurich, Rämistrasse 100, 8091 ZurichSwitzerland
  2. 2.Department of Pathology, University Hospital ZurichSwitzerland
  3. 3.Center for Radiopharmaceutical Sciences, University Hospital ZürichSwitzerland
  4. 4.Novartis Pharma Inc, BaselSwitzerland
  5. 5.Institute of Medical Microbiology, University Hospital ZurichSwitzerland

Personalised recommendations