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Environmental Geology

, 58:1567 | Cite as

Influence of acid mine drainage on microbial communities in stream and groundwater samples at Guryong Mine, South Korea

  • Jaisoo Kim
  • So-Yeon Koo
  • Ji-Young Kim
  • Eun-Hee Lee
  • Sang-Don Lee
  • Kyung-Seok Ko
  • Dong-Chan Ko
  • Kyung-Suk ChoEmail author
Original Article

Abstract

The effects of acid mine drainage (AMD) in a stream and groundwater near an abandoned copper mine were characterized by physicochemical properties, bacterial community structure using denaturing gel gradient electrophoresis (DGGE), and microbial activity/diversity using Ecoplate technique. Based on DGGE fingerprints, the eubacterial community structures grouped into the stream water (GRS1, GRS2 and GRS3) and groundwater samples (GW1 and GW2), apparently based on differences in water temperature and the concentrations of dissolved oxygen, nitrate and sulfate. The most highly AMD-contaminated sample (GRS1) had additional α-Proteobacteria whereas the groundwater samples included additional β-Proteobacteria, suggesting the development of populations resistant to AMD toxicity under aerobic and anaerobic conditions, respectively. Community level physiological activities on the 31 Ecoplate substrates suggested that the activities decreased with increasing concentrations of sulfate and heavy metals derived from AMD. The Shannon index showed that microbial diversity was greatest in GRS2, and lowest in GRS1, and was probably related to the level of AMD.

Keywords

Acid mine drainage Groundwater Stream Microbial community structure Microbial diversity 

Notes

Acknowledgments

This research was supported by a grant (project: Application of groundwater ecology to characterization of groundwater systems and contaminant behavior) from the Korea Research Council of Public Science & Technology. S. Y. Koo and J. Y. Kim were financially supported by the Korea Science and Engineering Foundation (KOSEF) through the Advanced Environmental Biotechnology Research Center at Pohang University of Science and Technology (R11-2003-006-06001-0). E. H. Lee was financially supported by the KOSEF NRL Program grant funded by the Korea government (MEST) (R0A-2008-000-20044-0).

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Jaisoo Kim
    • 1
  • So-Yeon Koo
    • 2
  • Ji-Young Kim
    • 2
  • Eun-Hee Lee
    • 2
  • Sang-Don Lee
    • 2
  • Kyung-Seok Ko
    • 3
  • Dong-Chan Ko
    • 3
  • Kyung-Suk Cho
    • 2
    Email author
  1. 1.Department of Life ScienceKyonggi UniversitySuwonSouth Korea
  2. 2.Department of Environmental Science and EngineeringEwha Womans UniversitySeoulSouth Korea
  3. 3.Ground and Geothermal Resources DivisionKorea Institute of Geoscience and Mineral ResourcesDaejeonSouth Korea

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