Applied Microbiology and Biotechnology

, Volume 56, Issue 3–4, pp 513–516

Coupling effects of osmotic pressure and temperature on the viability of Saccharomyces cerevisiae

  •  L. Beney
  •  P. Marechal
  •  P. Gervais
Short Contribution

Abstract.

The osmotic tolerance of cells of Saccharomyces cerevisiae as a function of glycerol concentration and temperature has been investigated. Results show that under isothermal conditions (25 °C) cells are resistant (94% viability) to hyperosmotic treatment at 49.2 MPa. A thigher osmotic pressure, cell viability decreases to 25% at 99 MPa. Yeast resistance to high osmotic stress (99 Mpa) is enhanced at low temperatures (5–11 °C). Therefore, the temperature at which hyperosmotic pressure is achieved greatly affects cell viability. These results suggest that temperature control is a suitable means of enhancing cell survival in response to osmotic dehydration.

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Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  •  L. Beney
    • 1
  •  P. Marechal
    • 1
  •  P. Gervais
    • 1
  1. 1.Laboratoire de Génie des Procédés Alimentaires et Biotechnologiques, Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et l'Alimentation, 1 Esplanade Erasme, 21000 Dijon, France

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