Applied Microbiology and Biotechnology

, Volume 53, Issue 5, pp 509–516 | Cite as

Three biotechnical processes using Ashbya gossypii, Candida famata, or Bacillus subtilis compete with chemical riboflavin production

  • K. -P. Stahmann
  • J. L. Revuelta
  • H. Seulberger


Chemical riboflavin production, successfully used for decades, is in the course of being replaced by microbial processes. These promise to save half the costs, reduce waste and energy requirements, and use renewable resources like sugar or plant oil. Three microorganisms are currently in use for industrial riboflavin production. The hemiascomycetes Ashbya gossypii, a filamentous fungus, and Candida famata, a yeast, are naturally occurring overproducers of this vitamin. To obtain riboflavin production with the Gram-positive bacterium Bacillus subtilis requires at least the deregulation of purine synthesis and a mutation in a flavokinase/FAD-synthetase. It is common to all three organisms that riboflavin production is recognizable by the yellow color of the colonies. This is an important tool for the screening of improved mutants. Antimetabolites like itaconate, which inhibits the isocitrate lyase in A. gossypii, tubercidin, which inhibits purine biosynthesis in C. famata, or roseoflavin, a structural analog of riboflavin used for B. subtilis, have been applied successfully for mutant selections. The production of riboflavin by the two fungi seems to be limited by precursor supply, as was concluded from feeding and gene-overexpression experiments. Although flux studies in B. subtilis revealed an increase both in maintenance metabolism and in the oxidative part of the pentose phosphate pathway, the major limitation there seems to be the riboflavin pathway. Multiple copies of the rib genes and promoter replacements are necessary to achieve competitive productivity.


Bacillus Subtilis Riboflavin Lyase Isocitrate Pentose Phosphate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • K. -P. Stahmann
    • 1
  • J. L. Revuelta
    • 2
  • H. Seulberger
    • 3
  1. 1.Institut für Biotechnologie 1, Forschungszentrum Jülich, 52425 Jülich, Germany e-mail: Tel.: +49-2461-612843/5446 Fax: +49-2461-612710DE
  2. 2.Departamento de Microbiologia y Genetica, Universidad de Salamanca, Campus Miguel de Unamuno, 37007 Salamanca, SpainES
  3. 3.SunGene, Corrensstrasse 3, 06466 Gatersleben, GermanyDE

Personalised recommendations