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Applied Microbiology and Biotechnology

, Volume 53, Issue 1, pp 69–74 | Cite as

Isolation and characterisation of a ropy Lactobacillus strain producing the exopolysaccharide kefiran

  • L. Micheli
  • D. Uccelletti
  • C. Palleschi
  • V. Crescenzi
ORIGINAL PAPER

Abstract

A capsular-polysaccharide-producing strain, LM-17, was isolated from kefir grains and was identified as a slime-forming, rod-shaped Lactobacillus. According to 1H- and 13C-NMR spectral data, the exopolysaccharide produced by the isolated bacterial strain is identical to the glucogalactan extracted from kefir grains and therefore known as kefiran. The kefiran produced was characterised by means of viscosity, optical rotatory power, circular dichroism and IR spectral measurements. A batch procedure was set up for the culture and extraction of the exopolysaccharide in laboratory conditions, resulting in a yield of 2 g/l purified kefiran from the culture supernatant of the LM-17 strain.

Keywords

Viscosity Bacterial Strain Lactobacillus Laboratory Condition Culture Supernatant 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • L. Micheli
    • 1
  • D. Uccelletti
    • 2
  • C. Palleschi
    • 2
  • V. Crescenzi
    • 1
  1. 1.Department of Chemistry, University of Rome “La Sapienza”, Ple Aldo Moro 5, I-00185 Rome, Italy e-mail: crescenzi@axrma.uniromal.it Tel.: +390649913630IT
  2. 2.Department of Developmental and Cell Biology, University of Rome “La Sapienza”, Rome, ItalyIT

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