Applied Microbiology and Biotechnology

, Volume 52, Issue 3, pp 440–445 | Cite as

Continuous degradation of mixtures of 4-nitrobenzoate and 4-aminobenzoate by immobilized cells of Burkholderia cepacia strain PB4

  • C. M. Peres
  • B. Van Aken
  • H. Naveau
  • S. N. Agathos
ORIGINAL PAPER

Abstract

 Although isolated on 4-aminobenzoate, Burkholderia cepacia strain PB4 is also able to grow on 4-nitrobenzoate. Degradation of an equimolar mixture of the nitroaromatic compound 4-nitrobenzoate and its corresponding aminoaromatic derivative 4-aminobenzoate by this strain was investigated. Batch experiments showed that, irrespective of preculturing conditions, both compounds were degraded simultaneously. The mixture-degrading ability of B. cepacia strain PB4 was subsequently tested in continuous packed bed reactors (PBR) with the strain immobilized on Celite grade R-633 or R-635. Higher degradation rates were achieved with the larger particles of Celite R-635. Maximum simultaneous degradation rates per liter of packed bed of 0.925 mmol l−1 h−1 4-nitrobenzoate and 4-aminobenzoate were obtained for an applied loading rate of the same value (0.925 mmol l−1 h−1 of each compound). Even when the applied load was not removed in its entirety, neither of the two compounds was degraded preferentially but a percentage of both of them was mineralized. The present study shows the possibility for a pure strain to biodegrade not only a nitroaromatic compound (4-nitrobenzoate) but also its corresponding amino derivative (4-aminobenzoate) continuously and simultaneously.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • C. M. Peres
    • 1
  • B. Van Aken
    • 1
  • H. Naveau
    • 1
  • S. N. Agathos
    • 1
  1. 1.Unit of Bioengineering, Catholic University of Louvain, Place Croix du Sud 2/19, B-1348 Louvain-la-Neuve, Belgium e-mail: agathos@gebi.ucl.ac.be Tel.: +32-10-473655 Fax: +32-10-473062BE

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