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Applied Microbiology and Biotechnology

, Volume 51, Issue 6, pp 786–793 | Cite as

Isolation and characterization of indene bioconversion genes from Rhodococcus strain I24

  • S. L. Treadway
  • K. S. Yanagimachi
  • E. Lankenau
  • P. A. Lessard
  • G. Stephanopoulos
  • A. J. Sinskey
ORIGINAL PAPER

Abstract

Rhodococcus strain I24 is able to convert indene into indandiol via the actions of at least two dioxygenase systems and a putative monooxygenase system. We have identified a cosmid clone from I24 genomic DNA that is able to confer the ability to convert indene to indandiol upon Rhodococcus erythropolis SQ1, a strain that normally can not convert or metabolize indene. HPLC analysis reveals that the transformed SQ1 strain produces cis-(1R,2S)-indandiol, suggesting that the cosmid clone encodes a naphthalene-type dioxygenase. DNA sequence analysis of a portion of this clone confirmed the presence of genes for the dioxygenase as well as genes encoding a dehydrogenase and putative aldolase. These genes will be useful for manipulating indene bioconversion in Rhodococcus strain I24.

Keywords

Indene HPLC Sequence Analysis HPLC Analysis Rhodococcus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • S. L. Treadway
    • 1
  • K. S. Yanagimachi
    • 2
  • E. Lankenau
    • 1
  • P. A. Lessard
    • 1
  • G. Stephanopoulos
    • 2
  • A. J. Sinskey
    • 1
  1. 1.Department of Biology, Massachusetts Institute of Technology, Bldg. 68-370, 77 Massachusetts Avenue, Cambridge, MA 02139, USA e-mail: asinskey@mit.edu Tel.: +1-617-253-6721 Fax: +1-617-253-8550US
  2. 2.Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USAUS

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