Applied Microbiology and Biotechnology

, Volume 51, Issue 6, pp 703–710

Artificial redox coenzymes: biomimetic analogues of NAD+

  • R. J. Ansell
  • C. R. Lowe
MINI-REVIEW

Abstract

A range of biomimetic analogues of the nicotinamide nucleotide coenzymes NAD(P)(H) have been developed based on the structure of a triazine dye template. These biomimetic redox coenzymes are relatively straightforward and inexpensive to synthesise and display NAD+-like activity with different dehydrogenases, despite their apparently minimal structural similarity to the native coenzyme NAD+. Horse liver alcohol dehydrogenase oxidises butan-1-ol, using the most active biomimetic coenzyme (Nap 1), with a kcat value an order of magnitude lower and a Km for the coenzyme two orders of magnitude higher than those using native NAD+. The enzymatically reduced biomimetic coenzymes may be reoxidised by phenazine methosulfate. We believe that these coenzymes may find applications in biotransformations and biosensors, and in the development of biomimetic catalysts where the redox enzyme itself is replaced by a synthetic binding site.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • R. J. Ansell
    • 1
  • C. R. Lowe
    • 1
  1. 1.Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QT, UK e-mail: crl1@biotech.cam.ac.uk Tel.: +44 1223 334160 Fax: +44 1223 334162GB

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