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Applied Microbiology and Biotechnology

, Volume 51, Issue 2, pp 229–234 | Cite as

Bacterial growth in space flight: logistic growth curve parameters for Escherichia coli and Bacillus subtilis

  • M. A. Kacena
  • G. A. Merrell
  • B. Manfredi
  • E. E. Smith
  • D. M. Klaus
  • P. Todd
ORIGINAL PAPER

Abstract

Previous investigations have reported that bacterial suspension cultures grow to higher stationary concentrations in space flight than on Earth; however, none of these investigations included extensive ground controls under varied inertial conditions. This study includes extensive controls and cell-growth data taken at several times during lag phase, log phase, and stationary phase of Escherichia coli and Bacillus subtilis. The Marquardt-Levenberg, least-squares fitting algorithm was used to calculate kinetic growth parameters from the logistic bacterial growth equations for space-flight and control growth curves. Space-flight cultures grew to higher stationary-phase concentrations and had shorter lag-phase durations. Also, evidence was found for increased exponential growth rate in space.

Keywords

Growth Curve Bacterial Growth Bacillus Subtilis Space Flight Ground Control 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • M. A. Kacena
    • 1
  • G. A. Merrell
    • 1
  • B. Manfredi
    • 1
  • E. E. Smith
    • 1
  • D. M. Klaus
    • 1
  • P. Todd
    • 1
  1. 1.University of Colorado, BioServe Space Technologies, Engineering Center, ECAE 1B-01, Campus Box 429, Boulder, CO 80309-0429, USA e-mail: todd@spot.colorado.edu Tel.: +1-303-492-5936 Fax: +1-303-492-4341US

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