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Applied Microbiology and Biotechnology

, Volume 50, Issue 6, pp 697–703 | Cite as

Screening and characterization of Lactobacillus strains producing large amounts of exopolysaccharides

  • G. H. van Geel-Schutten
  • F. Flesch
  • B. ten Brink
  • M. R. Smith
  • L. Dijkhuizen
ORIGINAL PAPER

Abstract

A total of 182 Lactobacillus strains were screened for production of extracellular polysaccharides (EPS) by a new method: growth in liquid media with high sugar concentrations. Sixty EPS-positive strains were identified; 17 strains produced more than 100 mg/l soluble EPS. Sucrose was an excellent substrate for abundant EPS synthesis. The ability to produce glucans appears to be widespread in the genus Lactobacillus. The monosaccharide composition of EPS produced by Lactobacillus reuteri strain LB 121 varied with the growth conditions (solid compared to liquid medium) and the sugar substrates (sucrose or raffinose) supplied in the medium. Strain LB 121 produced both a glucan and a fructan on sucrose, but only a fructan on raffinose. This is the first report of fructan production by a Lactobacillus species. EPS production increased with increasing sucrose concentrations and involved extracellular sucrase-type enzymes.

Keywords

Lactobacillus Glucan Monosaccharide Raffinose Fructan 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • G. H. van Geel-Schutten
    • 1
  • F. Flesch
    • 1
  • B. ten Brink
    • 1
  • M. R. Smith
    • 3
  • L. Dijkhuizen
    • 2
  1. 1.TNO Nutrition and Food Research, Department of Bioprocessing, PO Box 106, 3700 AJ Zeist, The NetherlandsNL
  2. 2.Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, PO Box 14, 9750 AA Haren, The Netherlands Tel.: +31-50-363-2150 Fax: +31-50-363-2154NL
  3. 3.NIZO, Department of Microbiology, PO Box 20, 6710 BA Ede, The NetherlandsNL

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