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Applied Microbiology and Biotechnology

, Volume 49, Issue 5, pp 579–583 | Cite as

The effects of space flight on the production of monorden by Humicola fuscoatra WC5157 in solid-state fermentation

  • K. S. Lam
  • S. W. Mamber
  • E. J. Pack
  • S. Forenza
  • P. B. Fernandes
  • D. M. Klaus
ORIGINAL PAPER

Abstract

The effect of space flight on the production of the antibiotic monorden on two types of agar media, T8 and PG, by Humicola fuscoatra WC5157 was examined on board the US Space Shuttle mission STS-77 in May 1996. Paired space-flight and ground control samples were prepared using identical hardware, protocol, media, and inoculum. Inoculation occurred simultaneously for both groups 2.5 h after launch. The flight and ground samples were allowed to grow for the entire 10-day mission in a dark, thermally controlled (22 °C) environment. Post-flight HPLC analysis of the flight and ground sample extracts indicated that the production of monorden by H. fuscoatra WC5157 in the flight samples was higher than in the ground samples in both agar media. In the T8 medium, the production of monorden in the flight and ground samples was 11.6 ± 3.5 μg and 8.9 ± 1.1 μg respectively (30% increase). In the PG medium, the production of monorden in the flight and ground samples was 23.8 ± 3.3 μg and 8.2 ± 2.2 μg respectively (190% increase). The production of monorden in the flight and ground control samples was confirmed by HPLC-MS analysis.

Keywords

Fermentation Agar Medium Sample Extract Space Flight Ground Sample 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • K. S. Lam
    • 1
  • S. W. Mamber
    • 1
  • E. J. Pack
    • 1
  • S. Forenza
    • 1
  • P. B. Fernandes
    • 2
  • D. M. Klaus
    • 3
  1. 1.Bristol-Myers Squibb Pharmaceutical Research Institute, Natural Products Research, Wallingford, CT 06492, USA Tel.: +1 203 284 6380 Fax: +1 203 284 6417 e-mail: ray_lam@ccmail.bms.comUS
  2. 2.Small Molecule Therapeutics, Monmouth Junction, NJ 08852, USAUS
  3. 3.BioServe Space Technologies, Aerospace Engineering Sciences Department, University of Colorado, Boulder, CO 80309, USAUS

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