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Applied Microbiology and Biotechnology

, Volume 49, Issue 4, pp 353–358 | Cite as

Production of xanthan gum and ice-nucleating material from whey by Xanthomonas campestris pv. translucens

  • H. Kawahara
  • H. Obata
Original paper

Abstract

Xanthomonas campestris pv. translucens IFO13599 could produce xanthan gum (18.5 mg/100 mg, lactose) with lactose as the growth substrate in spite of a low level of β-galactosidase. This productivity corresponded to one-fifth that with glucose. This strain could also produce ice-nucleating material having an ice-nucleating temperature, T50, of −2.8 °C with xanthan gum in the culture broth. We found that this strain produced both materials in whey medium from which the insoluble components had been removed. The production of xanthan with ice-nucleating material reached a maximum after cultivation for 168 h under optimum conditions. Furthermore, the xanthan obtained had a low viscosity because of its variant structure revealed, by TLC and HPLC analyses, to be lacking pyruvic acid. Furthermore, we concluded that this mixture had considerable potential as a regeneratic agent, when compared to other regeneratic agents such as carboxymethylcellulose.

Keywords

Glucose Viscosity HPLC Optimum Condition Lactose 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • H. Kawahara
    • 1
  • H. Obata
    • 1
  1. 1.Department of Biotechnology, Faculty of Engineering, Kansai University, 3-3-35 Yamate-cho, Suita-shi, Osaka 564-8680, Japan Tel.: +81 6 368 0832 Fax: +81 6 388 8609 e-mail: kawahara@ipcku.kansai-u.ac.jpJP

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