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Applied Microbiology and Biotechnology

, Volume 49, Issue 3, pp 295–300 | Cite as

Peroxisomal β-oxidation activities and γ-decalactone production by the yeast Yarrowia lipolytica

  • Y. Pagot
  • A. Le Clainche
  • J.-M. Nicaud
  • Y. Wache
  • J.-M. Belin
ORIGINAL PAPER

Abstract

γ-Decalactone is a peachy aroma compound resulting from the peroxisomal β-oxidation of ricinoleic acid by yeasts. The expression levels of acyl-CoA oxidase (gene deletion) and 3-ketoacyl-CoA thiolase activities (gene amplification on replicative plasmids) were modified in the yeast Yarrowia lipolytica. The effects of these modifications on β-oxidation were measured. Overexpression of thiolase activity did not have any effect on the overall β-oxidation activity. The disruption of one of the acyl-CoA oxidase genes resulted in an enhanced activity. The enhancement led to an increase of overall β-oxidation activity but reduced the γ-decalactone production rates. This seemed to indicate a non-rate-limiting role for β-oxidation in the biotransformation of ricinoleic acid to γ-decalactone by the yeast Yarrowia lipolytica. All strains produced and then consumed γ-decalactone. We checked the ability of the different strains to consume γ-decalactone in a medium containing the lactone as sole carbon source. The consumption of the strain overexpressing acyl-CoA oxidase activity was higher than that of the wild-type strain. We␣concluded that peroxisomal β-oxidation is certainly involved in γ-decalactone catabolism by the yeast Y.␣lipolytica. The observed production rates probably depend on an equilibrium between production and consumption of the lactone.

Keywords

Production Rate Lactone Biotransformation Sole Carbon Source Gene Deletion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Y. Pagot
    • 1
  • A. Le Clainche
    • 2
  • J.-M. Nicaud
    • 2
  • Y. Wache
    • 1
  • J.-M. Belin
    • 1
  1. 1.Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et␣l'Alimentation, Laboratoire de Biotechnologie, 1 Esplanade Erasme, F-21000 Dijon, France Fax: +33 03 80 39 66 11 e-mail: jmbelin@u-bourgogne.frFR
  2. 2.Laboratoire de Génétique Moléculaire et Cellulaire, CNRS ERS 567, Institut National Agronomique Paris-Grignon, F-78850 Thiverval-Grignon, FranceFR

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