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Applied Microbiology and Biotechnology

, Volume 49, Issue 2, pp 175–181 | Cite as

Oat β-glucan and xylan hydrolysates as selective substrates for Bifidobacterium and Lactobacillus strains

  • J. Jaskari
  • P. Kontula
  • A. Siitonen
  • H. Jousimies-Somer
  • T. Mattila-Sandholm
  • K. Poutanen
ORIGINAL PAPER

Abstract

Novel oligomers that resist digestion in the upper gut were prepared from oat mixed-linked β-glucan and xylan by enzymatic hydrolysis with lichenase of Bacillus subtilis and xylanase of Trichoderma reesei respectively. The low-molecular-mass hydrolysis products of β-glucan and xylan were compared with fructooligomers and raffinose in their ability to provide growth substrates for probiotic (Lactobacillus and Bifidobacterium) and intestinal (Bacteroides, Clostridium and Escherichia coli) strains in vitro. A degradation profile of each carbohydrate and total sugar consumption were analysed with HPLC, and bacterial growth rate with an automatic turbidometer, the Bioscreen C system. β-Glucooligomers and xylooligomers both enhanced the growth of health-promoting probiotic strains as compared with intestinal bacterial growth, but not to a significant level. Raffinose stimulated the probiotic strains significantly, whereas fructooligomers induced high average growth for intestinal bacteria also.

Key words Oat β-Glucooligomers Xylooligomers Fructooligomers Raffinose Prebiotic 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • J. Jaskari
    • 1
  • P. Kontula
    • 1
  • A. Siitonen
    • 2
  • H. Jousimies-Somer
    • 2
  • T. Mattila-Sandholm
    • 1
  • K. Poutanen
    • 1
  1. 1.VTT Biotechnology and Food Research, P.O. Box 1500, FIN-02044 VTT, Finland Tel.: + 358 0 456 5192 Fax: + 358 0 455 2103 e-mail: Kaisa.Poutanen@vtt.fiFI
  2. 2.National Public Health Institute (KTL), Mannerheimintie 166, FIN-00300 Helsinki, FinlandFI

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