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Applied Microbiology and Biotechnology

, Volume 48, Issue 2, pp 232–235 | Cite as

One-step transformation of the dimorphic yeast Yarrowia lipolytica

  • D.-C. Chen
  • J.-M. Beckerich
  • C. Gaillardin
SHORT CONTRIBUTION

Abstract

An efficient one-step transformation method for the dimorphic yeast Yarrowia lipolytica is described. Using cells grown overnight on agar plates, the whole process is carried out within 1 h. The transformant clones could be recovered on selective plates as early as 36–48 h after plating. The efficiency was better than 105 transformants/μg replicative plasmid DNA. Effects of cell density, dithiothreitol, heat shock, poly(ethylene glycol) 4000 concentration and the wetness of selective plates were investigated.

Keywords

Ethylene Agar Ethylene Glycol Heat Shock Cell Density 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • D.-C. Chen
    • 1
  • J.-M. Beckerich
    • 1
  • C. Gaillardin
    • 1
  1. 1.Laboratoire de Genétique Moléculaire et Cellulaire, Institut National de la Recherche Agromique-CNRS, Centre de Biotechnologie Agro-Industrielle, Institut National Agronomique Paris-Grignon, F-78850, Thiverval-Grignon, Paris, France Tel.: (+33 1) 30815443 Fax: (+33 1) 30815457FR

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