Applied Microbiology and Biotechnology

, Volume 48, Issue 2, pp 184–190 | Cite as

Determination of the kinetic parameters during continuous cultivation of the lipase-producing thermophile Bacillus sp. IHI-91 on olive oil

  • P. Becker
  • I. Abu-Reesh
  • S. Markossian
  • G. Antranikian
  • H. Märkl
ORIGINAL PAPER

Abstract

A thermostable lipase was produced in continuous cultivation of a newly isolated thermophilic Bacillus sp. strain IHI-91 growing optimally at 65 °C. Lipase activity decreased with increasing dilution rate while lipase productivity showed a maximum of 340 U l−1 h−1 at a dilution rate of 0.4 h−1. Lipase productivity was increased by 50% compared to data from batch fermentations. Up to 70% of the total lipase activity measured was associated to cells and by-products or residual substrate. Kinetic and stoichiometric parameters for the utilisation of olive oil were determined. The maximal biomass output method led to a saturation constant KS of 0.88 g/l. Both batch growth data and a washout experiment yielded a maximal specific growth rate, μmax, of 1.0 h−1. Oxygen uptake rates of up to 2.9 g l−1h−1 were calculated and the yield coefficient, YX/O, was determined to be 0.29 g dry cell weight/g O2. From an overall material balance the yield coefficient, YX/S, was estimated to be 0.60 g dry cell weight/g olive oil.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • P. Becker
    • 1
  • I. Abu-Reesh
    • 3
  • S. Markossian
    • 2
  • G. Antranikian
    • 2
  • H. Märkl
    • 1
  1. 1.Institute of Bioprocess and Biochemical Engineering, Technical University of Hamburg-Harburg, Denickestr. 15, D-21071 Hamburg, Germany. Tel.: +44 40 77183017 Fax: +44 40 77182909DE
  2. 2.Institute of Technical Microbiology, Technical University of Hamburg-Harburg, Denickestr. 15, D-21071 Hamburg, Germany.DE
  3. 3.Faculty of Engineering and Technology, University of Jordan, Amman, Jordan.JO

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