Applied Microbiology and Biotechnology

, Volume 47, Issue 5, pp 543–546

Plasmids for efficient single-copy gene cloning into gdh2 and trpC of Bacillus megaterium DSM319 and QM B1551

  • D. Schmiedel
  • P. S. Vary
  • L. Jablonski
  • W. Hillen
SHORT CONTRIBUTION

DOI: 10.1007/s002530050970

Cite this article as:
Schmiedel, D., Vary, P., Jablonski, L. et al. Appl Microbiol Biotechnol (1997) 47: 543. doi:10.1007/s002530050970

Abstract

We constructed integrative plasmids to place xylA-lacZ indicator gene fusions into two different loci of the Bacillus megaterium chromosome, gdh2 and trpC, in lac mutants of strains DSM 319 and QM B1551, which differ markedly. Single-crossover integration was achieved in all cases while double crossovers occurred only in gdh2 of DSM 319 and QM B1551 and in trpC of QM B1551. Neither of the loci affected regulation of the xylA-lacZ fusions. These results confirm the suitability of the two gene loci for single-copy cloning.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • D. Schmiedel
    • 1
  • P. S. Vary
    • 2
  • L. Jablonski
    • 2
  • W. Hillen
    • 1
  1. 1.Lehrstuhl für Mikrobiologie, Institut für Mikrobiologie, Biochemie und Genetik der Friedrich-Alexander Universität Erlangen-Nürnberg, Staudtstr. 5, D-91058 Erlangen, Germany Fax: +49 9131 858082 e-mail: whillen@biologie.uni-erlangen.de.DE
  2. 2.Department of Biological Sciences, Northern Illinois University, DeKalb, Illinois 60115, USAUS

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