Applied Microbiology and Biotechnology

, Volume 47, Issue 3, pp 306–308

Catechol dioxygenase expression in a Pseudomonas fluorescens strain exposed to different aromatic compounds

  • G. Cenci
  • G. Caldini
SHORT CONTRIBUTION

Abstract

Batch cultures of Pseudomonas fluorescens (strain P2a) maintained under carbon-limiting conditions in the presence of chrysene and other aromatics, survived starvation with no detectable changes in cell number for at least 4 months. P2a also demonstrated high dioxygenase levels after growth on benzoate and catechol. To characterize this strain further, early stationary-phase cells were resuspended in fresh mineral medium containing different aromatics, to evaluate enzyme expression in the presence of high-molecular-mass (isocyclic and heterocyclic) compounds. Results demonstrated effects on catechol 1,2-dioxygenase modulation by the model compounds used, confirming a low substrate specificity for this enzyme. The increases of specific activity observed in the presence of heterocyclic compounds were higher than those observed with isocyclic compounds.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • G. Cenci
    • 1
  • G. Caldini
    • 1
  1. 1.Dipartimento di Biologia Cellulare e Molecolare, Università di Perugia, Via del Giochetto, I-06126 Perugia, Italy. Fax: +39 75 5853328IT

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