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Applied Microbiology and Biotechnology

, Volume 47, Issue 3, pp 207–211 | Cite as

Saponified palm kernel oil and its major free fatty acids as carbon substrates for the production of polyhydroxyalkanoates in Pseudomonas putida PGA1

  • I. K. P. Tan
  • K. Sudesh Kumar
  • M. Theanmalar
  • S. N. Gan
  • B. Gordon III.
ORIGINAL PAPER

Abstract

The synthesis of polyhydroxyalkanoates (PHA) by Pseudomonas putida PGA1, using saponified palm kernel oil (SPKO), was investigated. The PHA produced from SPKO was compared with those produced by the major free fatty acids found in the palm kernel oil. Owing to the absence of lipase activity in P.␣putida, palm kernel oil did not support cell growth. However, SPKO could support cell growth and produced relatively high yield of both dry cells and PHA. The polyester produced was similar in properties to those derived from lauric (C12:0) and myristic (C14:0) acids, while oleic acid (C18:1) gave rise to PHA that was sticky and of broader molecular mass distribution. Nuclear magnetic resonance and gas chromatography showed that these PHA were copolymers consisting mainly of n-alkanoate monomers ranging from C6 to C14, with C8 as the predominant component. PHA derived from SPKO and oleic acid also contained a small amount of unsaturated monomers.

Keywords

Chromatography Lipase Nuclear Magnetic Resonance Molecular Mass Oleic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • I. K. P. Tan
    • 3
  • K. Sudesh Kumar
    • 3
  • M. Theanmalar
    • 3
  • S. N. Gan
    • 1
  • B. Gordon III.
    • 2
  1. 1.Department of Chemistry, University of Malaya, 50603 Kuala Lumpur, MalaysiaMY
  2. 2.Polymer Program, PennState University, State College, PA 16081 USAUS
  3. 3.Institute of Advanced Studies, University of Malaya, 50603 Kuala Lumpur, Malaysia. Fax: +60 3 756 8940MY

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