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Applied Microbiology and Biotechnology

, Volume 46, Issue 5–6, pp 554–558 | Cite as

Construction of L-lysine-overproducing strains of Brevibacterium lactofermentum by targeted disruption of the hom and thrB genes

  • C. Fernández-González
  • J. A. Gil
  • L. M. Mateos
  • A. Schwarzer
  • A. Schäfer
  • J. Kalinowski
  • A. Pühler
  • J. F. Martín
SHORT CONTRIBUTION

Abstract

The mobilization of plasmids from gram-negative Escherichia coli to gram-positive Brevibacterium lactofermentum, mediated by P-type transfer functions, was used to construct disrupted mutants blocked specifically in the homoserine branch of the aspartate pathway. The mutant strain B. lactofermentum R31 showed an efficiency of conjugal transfer two to three orders of magnitude higher than that of the wild-type strain B.␣lactofermentum ATCC 13869. The hom- and thrB- disrupted mutants of B. lactofermentum ATCC 13869 were lysine overproducers. B. lactofermentum R31 mutants do not overproduce lysine because R31 is an alanine-overproducing strain and channels the pyruvate needed for lysine biosynthesis to the production of alanine.

Keywords

Escherichia Coli Aspartate Lysine Pyruvate Transfer Function 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • C. Fernández-González
    • 1
  • J. A. Gil
    • 1
  • L. M. Mateos
    • 1
  • A. Schwarzer
    • 2
  • A. Schäfer
    • 2
  • J. Kalinowski
    • 2
  • A. Pühler
    • 2
  • J. F. Martín
    • 1
  1. 1.Area de Microbiología, Departamento de Ecología, Genética y Microbiología, Facultad de Biología, Universidad de León, Spain. Fax: +34 87 291506ES
  2. 2.Department of Genetics, University of Bielefeld, P.O. Box 100131, D-33501 Bielefeld, GermanyDE

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